raw RNA-Seq data of Hemimastix kukwesjijk - SRA

SRX3182827: raw RNA-Seq data of Hemimastix kukwesjijk
1 ILLUMINA (Illumina MiSeq) run: 10M spots, 4.7G bases, 2.6Gb downloads

Design: Single Spironema and Hemimastix cells were isolated with drawn-out micropipettes, photodocumented by differential interference contrast (DIC) light microscopy (using a Zeiss Axiovert 200M / AxioCam ICc5 microscrope and camera system; Carl Zeiss AG, Germany), and subjected to Ôsingle-cellÕ transcriptomics using the Smart-seq2 protocol with modifications (Picelli et al 2014). Briefly, four (Spironema) or two (Hemimastix) cells were individually picked into 0.2% Triton-X lysis buffer, immediately frozen in liquid nitrogen, then thawed and re-frozen three times. The remaining procedure followed the original protocol, with 20 (Spironema) or 18 (Hemimastix) PCR cycles. cDNA quantity and quality was assessed i) by Qubit dsDNA HS assay (Thermo-Fisher, cat. #Q32851), and ii) by PCR, and cloning of cDNA fragments into StrataClone SoloPack competent cells (Agilent Technologies), with 12 clones each Sanger-sequenced. After library preparation with Illumina Nextera XT, sequencing was carried out on an Illumina MiSeq with 2x250Êbp dual reads, with the libraries multiplexed on the same run.

Submitted by: Dalhousie University

Study: Transcriptomes of Hemimastix kukwesjijk and Spironema cf. multiciliatum

PRJNA406960 • SRP117616 • All experiments • All runs

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Transcriptomes of Hemimastix kukwesjijk and Spironema cf. multiciliatum isolates, members of Hemimastigophora. Both Hemimastix and Spironema transcriptomes were generated with a single-cell protocol (Picelli et al 2014). Assembled transcriptomes were used for eukaryote-wide phylogenomic analyses of the group.

Sample: 2 isolated cells, used as input for single-cell transcriptomics

SAMN07634962 • SRS2511413 • All experiments • All runs

Organism: Hemimastix kukwesjijk

Library:

Name: Hemi_Hemimastigophora

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 10M spots, 4.7G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR6032744	10,025,019	4.7G	2.6Gb	2018-11-20

ID:: 4484780

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