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| Status |
Public on Aug 19, 2016 |
| Title |
The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Wingless (Wg)/Wnt signaling is conserved in all metazoan animals and plays critical roles in development. The Wg/Wnt morphogen reception is essential for signal activation, whose activity is mediated through the receptor complex and a scaffold protein Dishevelled (Dsh). We report here that the exon junction complex (EJC) activity is indispensable for Wg signaling by maintaining an appropriate level of Dsh protein for Wg ligand reception in Drosophila. Transcriptome analyses in Drosophila wing imaginal discs indicate that the EJC controls the splicing of the cell polarity gene disc large 1 (dlg1), whose coding protein directly interacts with Dsh. Genetic and biochemical experiments demonstrate that Dlg1 protein acts independently from its role in cell polarity to protect Dsh protein from lysosomal degradation. More importantly, human orthologous Dlg protein is sufficient to promote Dvl protein stabilization and Wnt signaling activity, thus revealing a conserved regulatory mechanism of Wg/Wnt signaling by Dlg and EJC.
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| Overall design |
whole transcriptome RNA-seq to examine mRNAs extracted from wildtype (i.e. overexpressing lacZ) and pre-EJC-defective (i.e. overexpressing tsu RNAi) wing discs, respectively.
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| Contributor(s) |
Liu M, Li Y, Li R, Li C, Zhu AJ |
| Citation(s) |
27536874 |
| |
| Submission date |
May 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruifeng Li |
| E-mail(s) |
lirf@pku.edu.cn
|
| Organization name |
Peking University
|
| Street address |
Haidian
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA320911 |
| SRA |
SRP074583 |
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