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| Status |
Public on Apr 22, 2016 |
| Title |
Hipposeq: an RNA-seq based atlas of gene expression in excitatory hippocampal neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we used next-generation RNA sequencing (RNA-seq) to produce a quantitative, whole genome atlas of gene expression for every excitatory neuronal class in the hippocampus; namely, granule cells and mossy cells of the dentate gyrus, and pyramidal cells of areas CA3, CA2, and CA1. Moreover, for the canonical neurons of the trisynaptic loop, we profiled transcriptomes at both dorsal and ventral poles, producing a cell class- and region-specific transcriptional atlas for these canonical populations.
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| Overall design |
Hippocampal RNA profiles were generated by deep sequencing on Illumina HiSeq 2500, with three biological replicates per population
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| Contributor(s) |
Cembrowski M, Spruston N |
| Citation(s) |
27113915 |
| |
| Submission date |
Nov 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Steven Cembrowski |
| Organization name |
University of British Columbia
|
| Department |
Cellular and Physiological Sciences
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| Lab |
Cembrowski
|
| Street address |
2350 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6S0B7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA302123 |
| SRA |
SRP066161 |
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