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| Status |
Public on Jun 08, 2016 |
| Title |
cis-Regulatory analysis of Ptch1 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Ptch1 is the primary receptor for Hedgehog ligands and functions as a negative regulator of the pathway. Ptch1 is upregulated in response to pathway activation and is a direct target of the Gli family of transcription factors that act as the downstream effectors of the pathwat. The goal of this study was to identify tissue specific Gli2 inputs to Ptch1 during development. Additionally, we assessed the prevalence of the repressive chromatin mark (H3K27me3) during directed differentiated of embryonic stem cells into neural progenitors.
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| |
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| Overall design |
Embryonic tissues were microdissected and pooled prior to formaldehyde fixation. ES cell derived neural progenitors were generated using embryoid body differentiation according to standard protocols.
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| Contributor(s) |
Peterson K, McMahon A |
| Citation(s) |
27146892 |
| |
| Submission date |
Jul 22, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Peterson |
| E-mail(s) |
kevin.peterson@med.usc.edu
|
| Phone |
323-442-8077
|
| Organization name |
Eli and Edythe Broad-CIRM Center for Regenerative Medicine, University of Southern California Keck School of Medicine
|
| Department |
Department of Stem Cell Biology and Regenerative Medicine
|
| Lab |
Andrew McMahon
|
| Street address |
1425 San Pablo St.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA290563 |
| SRA |
SRP061408 |
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