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| Status |
Public on Jun 11, 2014 |
| Title |
Comparison of gene expression profiles of miR-142-/- primary megakaryocytes and WT primary megakaryocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver cells were maintained in Dulbecco modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, 100µg/ml streptomycin, and 50ng/ml recombinant human thrombopoietin (TPO; Peprotech). After 5 days of culture, megakaryocytes were purified using a discontinuous bovine serum albumin gradient (BSA, Sigma–Aldrich; 3%, 1.5%, and 0%). Total RNA was isolated with Tri–Reagent (MRC) following manufacturer’s instructions, and its quality was assessed with ND–1000 Nanodrop (Peqlab) and on a 1.5% agarose gel.
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| Overall design |
Gene expression was measured in primary megakaryocytes cultured from miR-142-/- fetal livers and wild type fetal livers at E14.5. Two independent experiments were performed.
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| Contributor(s) |
Hornstein E, Chapnik E |
| Citation(s) |
24859754 |
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| Submission date |
Nov 06, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Elik Chapnik |
| E-mail(s) |
elik.chapnik@weizmann.ac.il
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| Organization name |
Weizmann Institute of Science
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| Department |
Molecular Genetics
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| Lab |
Eran Hornstein
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| Street address |
Herzel st.
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA227016 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52141_RAW.tar |
16.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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