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| Status |
Public on May 04, 2021 |
| Title |
Characterizing the tumor microenvironment of metastatic ovarian cancer by single cell transcriptomics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding the cellular composition of the tumor microenvironment and the interactions of the cells is essential to the development of successful immunotherapies in cancer. We perform single-cell RNA sequencing (scRNA-seq) of 9,885 cells isolated from the omentum in 6 patients with ovarian cancer and identify 9 major cell types, including cancer, stromal, and immune cells. Transcriptional analysis of immune cells stratifies our patient samples into 2 groups: (1) high T cell infiltration (high Tinf) and (2) low T cell infiltration (low Tinf). TOX-expressing resident memory CD8+ T (CD8+ Trm) and granulysin-expressing CD4+ T cell clusters are enriched in the high Tinf group. Concurrently, we find unique plasmablast and plasma B cell clusters, and finally, NR1H2+IRF8+ and CD274+ macrophage clusters, suggesting an anti-tumor response in the high Tinf group. Our scRNA-seq study of metastatic tumor samples provides important insights in elucidating the immune response within ovarian tumors.
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| |
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| Overall design |
scRNA-seq of ovarian cancer metastatic cells from six ovarian cancer patients
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| |
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| Contributor(s) |
Olalekan S, Xie B, Back R, Eckart H, Basu A |
| Citation(s) |
34038734 |
| |
| Submission date |
Mar 17, 2020 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Bingqing Xie |
| E-mail(s) |
bingqing.xie@utsouthwestern.edu
|
| Phone |
3127182092
|
| Organization name |
University of Texas Southwestern medical center
|
| Department |
Internal medicine
|
| Street address |
6001 Forest Park Rd
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75235 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA612966 |
| SRA |
SRP253041 |
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