RAD3 TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD3
Official Full Name: TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3
Primary source: SGD:S000000973
Locus tag: YER171W
See related: AllianceGenome:SGD:S000000973; FungiDB:YER171W; VEuPathDB:YER171W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: REM1
Summary: Enables 5'-3' DNA helicase activity and damaged DNA binding activity. Involved in nucleotide-excision repair; positive regulation of mitotic recombination; and transcription by RNA polymerase II. Part of nucleotide-excision repair factor 3 complex; transcription factor TFIIH core complex; and transcription factor TFIIH holo complex. Used to study Cockayne syndrome; photosensitive trichothiodystrophy; and xeroderma pigmentosum. Human ortholog(s) of this gene implicated in several diseases, including Cockayne syndrome (multiple); carcinoma (multiple); hematologic cancer (multiple); photosensitive trichothiodystrophy (multiple); and xeroderma pigmentosum (multiple). Orthologous to human ERCC2 (ERCC excision repair 2, TFIIH core complex helicase subunit). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RAD3 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (527082..529418)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Both rad3-101 and rad3-102 elevate the frequency of reciprocal crossovers about 100-fold. Mapping of these events shows that three-quarters of these crossovers reflect DSBs formed at the same positions in both sister chromatids
Title: High-Resolution Mapping of Homologous Recombination Events in rad3 Hyper-Recombination Mutants in Yeast.
When the Rad3 ATP-binding groove is mutated, TFIIH gains a higher affinity for DNA.
Title: The rem mutations in the ATP-binding groove of the Rad3/XPD helicase lead to Xeroderma pigmentosum-Cockayne syndrome-like phenotypes.
In mms19 mutant cells, protein levels of the core TFIIH component Rad3 and Ssl2 were significantly reduced
Title: Mms19 protein functions in nucleotide excision repair by sustaining an adequate cellular concentration of the TFIIH component Rad3.
rad3-102, a mutant allele of the gene RAD3, may increase lesions that elicit a response by the recombination machinery or, alternatively, stabilize recombinagenic lesions generated by DNA replication failure
Title: A mutant allele of the transcription factor IIH helicase gene, RAD3, promotes loss of heterozygosity in response to a DNA replication defect in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 5'-3' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables damaged DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of nucleotide-excision repair factor 3 complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIH core complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIH holo complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3

NP_011098.3

EC 3.6.4.12

5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

527082..529418

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179061.3 → NP_011098.3 TPA: TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011098.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM79, P06839

UniProtKB/TrEMBL

A6ZRC7, B3LRT0, B5VHV1, C7GS39, C8Z7J4, G2WD57, N1P571

Conserved Domains (1) summary

TIGR00604
Location:7 → 717

rad3; DNA repair helicase (rad3)