RFA2 Rfa2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RFA2
Official Full Name: Rfa2p
Primary source: SGD:S000005256
Locus tag: YNL312W
See related: AllianceGenome:SGD:S000005256; FungiDB:YNL312W; VEuPathDB:YNL312W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BUF1; RPA2; RPA32
Summary: Enables double-stranded DNA binding activity; sequence-specific DNA binding activity; and single-stranded DNA binding activity. Involved in several processes, including DNA metabolic process; DNA unwinding involved in DNA replication; and protein ubiquitination. Located in chromosome, telomeric region and condensed nuclear chromosome. Part of DNA replication factor A complex. Orthologous to several human genes including RPA2 (replication protein A2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RFA2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 2

Sequence:: Chromosome: XIV; NC_001146.8 (48287..49216)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

forms part of Replication Protein A (RPA) heterotrimer; dynamic DNA-binding domains promote the cooperative assembly of multiple RPAs on long ssDNA
Title: A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA.
Post-translational modification of the Rfa2 N-terminus is not required for cells to deal with DNA damage; however, it might influence whether cells proceed into M-phase in the continued presence of unrepaired DNA lesions.
Title: The DNA damage response and checkpoint adaptation in Saccharomyces cerevisiae: distinct roles for the replication protein A2 (Rfa2) N-terminus.
Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus.
Title: The DNA damage response at eroded telomeres and tethering to the nuclear pore complex.
Results suggests that RPA2 is necessary for structural integrity and may also be needed for intra- and/or inter-protein interactions.
Title: Essential functions of the 32 kDa subunit of yeast replication protein A.
Rfa2 serine 27 (S27) is required for Ime2-dependent Rfa2 phosphorylation in vivo.
Title: Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heteroduplex formation	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic recombination	IPI Inferred from Physical Interaction more info	PubMed
involved_in nucleotide-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleotide-excision repair	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IPI Inferred from Physical Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IPI Inferred from Physical Interaction more info	PubMed
involved_in telomere maintenance via recombination	IPI Inferred from Physical Interaction more info	PubMed
involved_in telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via telomere lengthening	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication factor A complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DNA replication factor A complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, telomeric region	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome, telomeric region	IMP Inferred from Mutant Phenotype more info	PubMed
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in site of double-strand break	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Rfa2p

NP_014087.1

Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

48287..49216

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183150.1 → NP_014087.1 TPA: Rfa2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014087.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0N3, P26754, P38905

UniProtKB/TrEMBL

A6ZSG8, B3LPE6, C7GWQ9, C8ZGL2, G2WLF7, N1NZC3

Conserved Domains (1) summary

COG5235
Location:1 → 273

RFA2; Single-stranded DNA-binding replication protein A (RPA), medium (30 kD) subunit [DNA replication, recombination, and repair]