ARP2 actin-related protein 2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ARP2
Official Full Name: actin-related protein 2
Primary source: SGD:S000002187
Locus tag: YDL029W
See related: AllianceGenome:SGD:S000002187; FungiDB:YDL029W; VEuPathDB:YDL029W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ACT2
Summary: Enables ATP binding activity and ATP hydrolysis activity. Involved in several processes, including Arp2/3 complex-mediated actin nucleation; ascospore wall assembly; and mitochondrion inheritance. Part of Arp2/3 protein complex. Colocalizes with mitochondrion. Orthologous to human ACTR2 (actin related protein 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ARP2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (399340..400638)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ARP2 role in the actin filament nucleation.
Title: Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.
These studies provide evidence that WASP (Las17) and WIP (Vrp1) play central roles in establishment of a robust multivalent Src homology 3 domain-proline-rich motif network in vivo, giving Actin-related protein 2/3 complex assembly onset at endocytic sites a switch-like behavior.
Title: Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo.
WASP displaces the autoinhibitory Arp3 C-terminal tail from a hydrophobic groove at Arp3's barbed end to destabilize the inactive state, providing a mechanism by which WASP stimulates the short-pitch conformation and activates the Arp2/3 complex.
Title: Role and structural mechanism of WASP-triggered conformational changes in branched actin filament nucleation by Arp2/3 complex.
Studies indicate that the WASP homologue Las17 is able to nucleate actin independently of Arp2/3 through the function of G-actin-binding activities in its polyproline region.
Title: WASP family proteins, more than Arp2/3 activators.
data does not support the simple hypothesis that the primary role for Arp2/3 regulators is to recruit and activate Arp2/3; rather data suggests that these regulators may be playing more subtle roles in establishing functional networks in vivo
Title: Molecular analysis of Arp2/3 complex activation in cells.
development of a direct tethering recruitment model for activation of Arp2/3 complex by Crn1
Title: Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.
These data identify Syp1 as a novel negative regulator of WASp-Arp2/3 complex that helps choreograph the precise timing of actin assembly during endocytosis.
Title: The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.
Results identify an N-terminal fragment of Las17p able to interact with Vrp1p but not Arp2/3, and suggest that LAs17 interaction with Vrp1 is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast.
Title: Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast.
An actin-related protein, Arp2, briefly colocalizes with Atg9 and directly regulates the dynamics of Atg9 movement.
Title: Arp2 links autophagic machinery with the actin cytoskeleton.
These findings reveal that Arp2p also mediates what appears to be an auto-regulatory mechanism possibly adapted for efficient coordination of actin assembly and disassembly during endocytosis.
Title: A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
contributes_to actin filament binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cortical patch organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ascospore wall assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitochondrion localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Arp2/3 protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Arp2/3 protein complex	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular non-membrane-bounded organelle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: actin-related protein 2

NP_010255.1

Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants