SAW1 DNA-binding protein SAW1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SAW1
Official Full Name: DNA-binding protein SAW1
Primary source: SGD:S000000025
Locus tag: YAL027W
See related: AllianceGenome:SGD:S000000025; FungiDB:YAL027W; VEuPathDB:YAL027W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 3'-flap-structured DNA binding activity and 5'-flap-structured DNA binding activity. Involved in double-strand break repair via single-strand annealing, removal of nonhomologous ends and positive regulation of endodeoxyribonuclease activity. Located in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SAW1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (94687..95472)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the combination of dynamic light scattering and differential scanning fluorimetry techniques to optimize the stability and homogeneity of recombinant Saw1. The protein expression and purification conditions identified in this study allow for higher recovery of soluble Saw1 and enable the biochemical characterization of the protein.
Title: Large-scale production of recombinant Saw1 in Escherichia coli.
results support a model in which Saw1 and Rad1-Rad10 are recruited as a complex to short and long flaps in all phases of cell cycle, but that Saw1 is only required for recruitment of Rad1-Rad10 to DSBs when long flaps are formed in G1
Title: Saw1 localizes to repair sites but is not required for recruitment of Rad10 to repair intermediates bearing short non-homologous 3' flaps during single-strand annealing in S. cerevisiae.
Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
Title: A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions.
Saw1 is required for Synthesis Dependent Strand Annealing double-strand break repair in S. cerevisiae.
Title: SAW1 is required for SDSA double-strand break repair in S. cerevisiae.
Saw1 plays a role in Rad1/Rad10 complex assembly at recombination intermediates in budding yeast.
Title: Role of Saw1 in Rad1/Rad10 complex assembly at recombination intermediates in budding yeast.
Data suggest that Saw1 targets Rad1/Rad10 to Rad52-coated recombination intermediates.
Title: Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-flap-structured DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables 5'-flap-structured DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables flap-structured DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding protein SAW1

NP_009375.1

5'- and 3'-flap DNA binding protein; required for recruitment of Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; forms a complex with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001133.9 Reference assembly

Range

94687..95472

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178172.1 → NP_009375.1 TPA: DNA-binding protein SAW1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009375.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPJ1, P39735

UniProtKB/TrEMBL

B3LUW1, B5VDJ3, C7GPK8, C8Z3J9, N1P927

Conserved Domains (1) summary

pfam11561
Location:1 → 243

Saw1; Single strand annealing-weakened 1