PWD chloroplastidic phosphoglucan, water dikinase (ATGWD3) [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PWD
Official Full Name: chloroplastidic phosphoglucan, water dikinase (ATGWD3)
Primary source: TAIR:AT5G26570
Locus tag: AT5G26570
See related: Araport:AT5G26570
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATGWD3; F9D12.1; F9D12_1; OK1; PHOSPHOGLUCAN WATER DIKINASE
Summary: chloroplastidic phosphoglucan, water dikinase (PWD) which is required for normal degradation of leaf starch in Arabidopsis. NMR analysis of the mutants, suggests that the gene is specifically involved in the phosphorylation of the glucosyl residues of starch at the C3 position.
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Genomic context

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See PWD in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 19

Sequence:: Chromosome: 5; NC_003076.8 (9261479..9267732)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our systematic study of the three GWDs of A. thaliana demonstrates that cytosolic GWD2, and in part also plastidic PWD, play unexpected roles in proper seed development.
Title: The analysis of the different functions of starch-phosphorylating enzymes during the development of Arabidopsis thaliana plants discloses an unexpected role for the cytosolic isoform GWD2.
The role of alpha-glucan/water dikinase in the development of cold tolerance in conjunction with SEX1 protein is reported.
Title: Starch-related alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in Arabidopsis.
The expression and characterization of this phosphoglucan,water dikinase in A. thaliana.
Title: Identification of a novel enzyme required for starch metabolism in Arabidopsis leaves. The phosphoglucan, water dikinase.
An Arabidopsis thaliana gene encoding a homologue of the potato alpha-glucan, water dikinase GWD, previously known as R1, was identified by screening the Arabidopsis genome and named AtGWD3. [GWD3]
Title: A novel isoform of glucan, water dikinase phosphorylates pre-phosphorylated alpha-glucans and is involved in starch degradation in Arabidopsis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001125808.1

Supported by ESTs:

NM_122538.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables carbohydrate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoglucan, water dikinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoglucan, water dikinase activity	IEA Inferred from Electronic Annotation more info
enables phosphoglucan, water dikinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables starch binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within starch catabolic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within starch metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed
located_in plastid	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: chloroplastidic phosphoglucan, water dikinase (ATGWD3)

NP_001119280.1

PHOSPHOGLUCAN WATER DIKINASE (PWD); FUNCTIONS IN: carbohydrate kinase activity, catalytic activity, phosphoglucan, water dikinase activity; INVOLVED IN: protein amino acid autophosphorylation, carbohydrate metabolic process, starch metabolic process, starch catabolic process; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Immunoglobulin-like fold (InterPro:IPR013783), Carbohydrate-binding-like fold (InterPro:IPR013784), Glycoside hydrolase, carbohydrate-binding (InterPro:IPR002044); BEST Arabidopsis thaliana protein match is: Pyruvate phosphate dikinase, PEP/pyruvate binding domain (TAIR:AT1G10760.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_198009.3

ATGWD3; FUNCTIONS IN: carbohydrate kinase activity, catalytic activity, phosphoglucan, water dikinase activity; INVOLVED IN: protein amino acid autophosphorylation, carbohydrate metabolic process, starch metabolic process, starch catabolic process; LOCATED IN: chloroplast stroma, chloroplast, plastid; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyruvate phosphate dikinase, PEP/pyruvate-binding (InterPro:IPR002192), Immunoglobulin-like fold (InterPro:IPR013783), Carbohydrate-binding-like fold (InterPro:IPR013784), Glycoside hydrolase, carbohydrate-binding (InterPro:IPR002044), ATP-grasp fold, subdomain 1 (InterPro:IPR013815); BEST Arabidopsis thaliana protein match is: Pyruvate phosphate dikinase, PEP/pyruvate binding domain (TAIR:AT1G10760.1); Has 3898 Blast hits to 3880 proteins in 1425 species: Archae - 252; Bacteria - 2881; Metazoa - 32; Fungi - 198; Plants - 278; Viruses - 0; Other Eukaryotes - 257 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

9261479..9267732

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_122538.5 → NP_198009.3 chloroplastidic phosphoglucan, water dikinase (ATGWD3) [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_198009.3

Status: REVIEWED

UniProtKB/Swiss-Prot

O81504, O81505, Q5XMK9, Q6ZY51, Q84TI8

UniProtKB/TrEMBL

A0A178UG93, A0A654G4K3

Conserved Domains (2) summary

cd05818
Location:73 → 164

CBM20_water_dikinase; Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the ...

pfam01326
Location:887 → 1194

PPDK_N; Pyruvate phosphate dikinase, PEP/pyruvate binding domain
NM_001125808.1 → NP_001119280.1 chloroplastidic phosphoglucan, water dikinase (ATGWD3) [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001119280.1

Status: REVIEWED

UniProtKB/TrEMBL

B3H4K8

Conserved Domains (1) summary

cd05818
Location:73 → 164

CBM20_water_dikinase; Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the ...