PGSIP1 plant glycogenin-like starch initiation protein 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PGSIP1
Official Full Name: plant glycogenin-like starch initiation protein 1
Primary source: TAIR:AT3G18660
Locus tag: AT3G18660
See related: Araport:AT3G18660
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: glucuronic acid substitution of xylan 1; GUX1; plant glycogenin-like starch initiation protein 1
Summary: Plants expressing an RNAi construct specifically targeting PGSIP1 was shown to have a dramatically reduced amount of starch. Encodes a glucuronyltransferase responsible for the addition of GlcA residues onto xylan and for secondary wall deposition.
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Genomic context

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See PGSIP1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 5

Sequence:: Chromosome: 3; NC_003074.8 (6419013..6422141, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GUX1 and GUX2 are required for different patterns of 4-O-methylglucuronic acid substitution of xylan.
Title: GUX1 and GUX2 glucuronyltransferases decorate distinct domains of glucuronoxylan with different substitution patterns.
GUX2 and GUX4, have activity as xylan alpha-glucuronosyltransferases.
Title: Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases.
GUX1/2/3 are glucuronyltransferases responsible for the glucuronic acid substitution of xylan, which is essential for normal secondary wall deposition and plant development.
Title: Arabidopsis GUX proteins are glucuronyltransferases responsible for the addition of glucuronic acid side chains onto xylan.
Data show that a mutant in the PGSIP1 gene encoding one of the Golgi localized candidate proteins has a highly decreased content of glucuronic acid in secondary cell walls and substantially reduced xylan glucuronosyltransferase activity.
Title: An integrative approach to the identification of Arabidopsis and rice genes involved in xylan and secondary wall development.
Data identify two Golgi-localized glycosyltransferases, GlucUronic acid substitution of Xylan (GUX)-1 and GUX2, that are required for addition of glucuronic acid and 4-O-methylglucuronic acid branches to xylan in Arabidopsis cell walls. [GUX1]
Title: Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001035645.3

Supported by ESTs:

NM_001202998.1

Supported by ESTs:

NM_112752.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables glucuronosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glucuronoxylan glucuronosyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within glucuronoxylan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within plant-type secondary cell wall biogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within starch metabolic process	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within xylan biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within xylan biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: plant glycogenin-like starch initiation protein 1

NP_001030722.1

plant glycogenin-like starch initiation protein 1 (PGSIP1); FUNCTIONS IN: transferase activity, transferring glycosyl groups; INVOLVED IN: biosynthetic process, starch metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 8 (InterPro:IPR002495); BEST Arabidopsis thaliana protein match is: plant glycogenin-like starch initiation protein 2 (TAIR:AT1G77130.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001189927.1

plant glycogenin-like starch initiation protein 1 (PGSIP1); FUNCTIONS IN: transferase activity, transferring glycosyl groups; INVOLVED IN: biosynthetic process, starch metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 8 (InterPro:IPR002495); BEST Arabidopsis thaliana protein match is: plant glycogenin-like starch initiation protein 2 (TAIR:AT1G77130.1).

NP_566615.1

plant glycogenin-like starch initiation protein 1 (PGSIP1); FUNCTIONS IN: transferase activity, transferring glycosyl groups; INVOLVED IN: biosynthetic process, starch metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 8 (InterPro:IPR002495); BEST Arabidopsis thaliana protein match is: plant glycogenin-like starch initiation protein 2 (TAIR:AT1G77130.1); Has 1355 Blast hits to 1348 proteins in 306 species: Archae - 0; Bacteria - 140; Metazoa - 259; Fungi - 278; Plants - 507; Viruses - 75; Other Eukaryotes - 96 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

6419013..6422141 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001035645.3 → NP_001030722.1 plant glycogenin-like starch initiation protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001030722.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q940B5, Q9LSB1

UniProtKB/TrEMBL

A0A654F9S6, W8Q6S4

Conserved Domains (2) summary

cd02537
Location:322 → 567

GT8_Glycogenin; Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen

COG1442
Location:397 → 533

RfaJ; Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis]
NM_001202998.1 → NP_001189927.1 plant glycogenin-like starch initiation protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189927.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A654F9S6, F4J8T8

Conserved Domains (2) summary

cd02537
Location:317 → 562

GT8_Glycogenin; Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen

COG1442
Location:392 → 528

RfaJ; Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis]
NM_112752.3 → NP_566615.1 plant glycogenin-like starch initiation protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_566615.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A654F9S6

Conserved Domains (2) summary

cd02537
Location:318 → 563

GT8_Glycogenin; Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen

COG1442
Location:393 → 529

RfaJ; Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis]