U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination
    • Showing Current items.

    JAR1 Auxin-responsive GH3 family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 819244, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    JAR1
    Official Full Name
    Auxin-responsive GH3 family protein
    Primary source
    TAIR:AT2G46370
    Locus tag
    AT2G46370
    See related
    Araport:AT2G46370
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtGH3.11; F11C10.6; FAR-RED INSENSITIVE 219; FIN219; JASMONATE RESISTANT 1
    Summary
    Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See JAR1 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    5
    Sequence:
    Chromosome: 2; NC_003071.7 (19033586..19036683)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene SPA (suppressor of phyA-105) protein family Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene extra-large G-like protein, putative (DUF3133) Neighboring gene succinate dehydrogenase subunit

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. JASMONATE RESISTANT 1 negatively regulates root growth under boron deficiency in Arabidopsis. Huang Y, et al. J Exp Bot, 2021 Apr 2. PMID 33530106
    2. Far-Red Light-Mediated Seedling Development in Arabidopsis Involves FAR-RED INSENSITIVE 219/JASMONATE RESISTANT 1-Dependent and -Independent Pathways. Chen HJ, et al. PLoS One, 2015. PMID 26176841, Free PMC Article
    3. The role of JAR1 in Jasmonoyl-L: -isoleucine production during Arabidopsis wound response. Suza WP, et al. Planta, 2008 May. PMID 18247047
    4. Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1). Guranowski A, et al. FEBS Lett, 2007 Mar 6. PMID 17291501
    5. Solar ultraviolet-B radiation alters the attractiveness of Arabidopsis plants to diamondback moths (Plutella xylostella L.): impacts on oviposition and involvement of the jasmonic acid pathway. Caputo C, et al. Oecologia, 2006 Aug. PMID 16639567

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. FAR-RED INSENSITIVE 219 and phytochrome B corepress shade avoidance via modulating nuclear speckle formation.
      Title: FAR-RED INSENSITIVE 219 and phytochrome B corepress shade avoidance via modulating nuclear speckle formation.
    2. JASMONATE RESISTANT 1 negatively regulates root growth under boron deficiency in Arabidopsis.
      Title: JASMONATE RESISTANT 1 negatively regulates root growth under boron deficiency in Arabidopsis.
    3. FIN219/JAR1 and CRY1 antagonize each other to modulate photomorphogenic development of seedlings and stress responses in Arabidopsis.FIN219 and CRY1 negatively regulated each other by direct interaction in response to jasmonate under blue light.
      Title: FIN219/JAR1 and cryptochrome1 antagonize each other to modulate photomorphogenesis under blue light in Arabidopsis.
    4. We show that the rotated C-terminal domain of FIN219 alters ATP binding and the core structure of the active site. We further demonstrate that this unique FIN219-FIP1 structure is crucial for increasing FIN219 activity and determines the priority of substrate binding.
      Title: Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
    5. FIN219/JAR1 is tightly regulated in response to exogenous jasmonic acid.
      Title: Far-Red Light-Mediated Seedling Development in Arabidopsis Involves FAR-RED INSENSITIVE 219/JASMONATE RESISTANT 1-Dependent and -Independent Pathways.
    6. crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1
      Title: Structural basis for prereceptor modulation of plant hormones by GH3 proteins.
    7. The regulation of COP1 by FIN219, was studied.
      Title: FAR-RED INSENSITIVE219 modulates CONSTITUTIVE PHOTOMORPHOGENIC1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis.
    8. Increase in jasmonic acid-isoleucine mediated by the JAR1 enzyme plays only a minor role in transcriptional modulation of genes induced by mechanical wounding.
      Title: The role of JAR1 in Jasmonoyl-L: -isoleucine production during Arabidopsis wound response.
    9. FIN219 interacts with FIP1 to regulate cell elongation and flowering in response to light.
      Title: Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis.
    10. analyzed the substrate specificity of recombinant JAR1 and determined whether it catalyzes the synthesis of mono- and dinucleoside polyphosphates
      Title: Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1).
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    NM_001084600.1
    NM_001202828.1
    NM_130200.2
    NM_180122.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables L-leucine binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables adenylyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables amino acid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables catalytic activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables jasmonoyl-L-amino acid ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables jasmonoyl-L-amino acid ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to auxin stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within induced systemic resistance, jasmonic acid mediated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in induced systemic resistance, jasmonic acid mediated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within jasmonic acid and ethylene-dependent systemic resistance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within jasmonic acid metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within jasmonic acid metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of defense response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within photomorphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein adenylylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within red, far-red light phototransduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of reactive oxygen species metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of response to red or far red light IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of stomatal movement IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to UV-B IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to jasmonic acid IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to mycotoxin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to ozone IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to wounding IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within systemic acquired resistance IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  
    located_in vacuole HDA PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    Auxin-responsive GH3 family protein
    NP_001078069.1
    • JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1356 Blast hits to 1352 proteins in 227 species: Archae - 0; Bacteria - 427; Metazoa - 52; Fungi - 2; Plants - 657; Viruses - 0; Other Eukaryotes - 218 (source: NCBI BLink).
    NP_001189757.1
    • JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1).
    NP_566071.1
    • JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).
    NP_850453.1
    • JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      19033586..19036683
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_180122.4 → NP_850453.1  Auxin-responsive GH3 family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850453.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      C0Z3D1, Q42147, Q56YY1, Q949V9, Q9LKI2, Q9SKE2
      UniProtKB/TrEMBL
      A0A178VQG1, A0A5S9X7G2, A0A7G2EKT2
      Conserved Domains (1) summary
      pfam03321
      Location:16 → 555
      GH3; GH3 auxin-responsive promoter

    2. NM_001202828.1 → NP_001189757.1  Auxin-responsive GH3 family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001189757.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2EKT2, F4II77
      Conserved Domains (2) summary
      cl21606
      Location:20 → 583
      PLN02247; indole-3-acetic acid-amido synthetase
      pfam03321
      Location:27 → 566
      GH3; GH3 auxin-responsive promoter

    3. NM_130200.2 → NP_566071.1  Auxin-responsive GH3 family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_566071.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      C0Z3D1, Q42147, Q56YY1, Q949V9, Q9LKI2, Q9SKE2
      UniProtKB/TrEMBL
      A0A178VQG1, A0A5S9X7G2, A0A7G2EKT2
      Conserved Domains (1) summary
      pfam03321
      Location:16 → 555
      GH3; GH3 auxin-responsive promoter

    4. NM_001084600.1 → NP_001078069.1  Auxin-responsive GH3 family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001078069.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A7G2EKT2
      Conserved Domains (2) summary
      cl21606
      Location:3 → 496
      PLN02247; indole-3-acetic acid-amido synthetase
      pfam03321
      Location:1 → 476
      GH3; GH3 auxin-responsive promoter
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SKE2.2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools