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    Marchf8 membrane associated ring-CH-type finger 8 [ Mus musculus (house mouse) ]

    Gene ID: 71779, updated on 11-Nov-2024

    Summary

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    Official Symbol
    Marchf8provided by MGI
    Official Full Name
    membrane associated ring-CH-type finger 8provided by MGI
    Primary source
    MGI:MGI:1919029
    See related
    Ensembl:ENSMUSG00000025702 AllianceGenome:MGI:1919029
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Mir; March8; MARCH-VIII; 1300017E09Rik
    Summary
    The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
    Expression
    Ubiquitous expression in kidney adult (RPKM 18.7), liver E14 (RPKM 18.4) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Marchf8 in Genome Data Viewer
    Location:
    6 E3; 6 53.78 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (116312546..116386501)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (116337599..116409540)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene WASH complex subunit 2 Neighboring gene predicted gene, 52873 Neighboring gene STARR-positive B cell enhancer ABC_E2799 Neighboring gene zinc finger, AN1-type domain 4 Neighboring gene STARR-seq mESC enhancer starr_17102 Neighboring gene predicted gene, 23527 Neighboring gene predicted gene, 38880 Neighboring gene arachidonate 5-lipoxygenase Neighboring gene olfactory receptor family 13 subfamily A member 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Anti-arthritic effect of E3 ubiquitin ligase, c-MIR, expression in the joints. Toyomoto M, et al. Int Immunol, 2011 Mar. PMID 21393633
    2. MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes. Liu X, et al. Nat Commun, 2021 Jul 20. PMID 34285233, Free PMC Article
    3. Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection. Liu H, et al. J Exp Med, 2016 Aug 22. PMID 27503069, Free PMC Article
    4. Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83. von Rohrscheidt J, et al. J Exp Med, 2016 Aug 22. PMID 27503071, Free PMC Article
    5. MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation. Yang X, et al. Sci Signal, 2022 May 3. PMID 35503863

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
      Title: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
    2. MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
      Title: MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
    3. MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
      Title: MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
    4. CD83-mediated MHCII stabilization through antagonism of March8 as a novel functional adaptation of cortical epithelial cells for T cell selection.
      Title: Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83.
    5. orchestrated regulation of MHC II surface expression in thymic epithelial cells by MARCH 8 and CD83 plays a major role in CD4(+) T cell selection.
      Title: Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables MHC class II protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables MHC protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin-protein transferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in adaptive immune response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in antigen processing and presentation of peptide antigen via MHC class II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in antigen processing and presentation of peptide antigen via MHC class II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in immune response IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of MHC class II biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein polyubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein polyubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in early endosome membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in early endosome membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endosome ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in late endosome membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in lysosomal membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in lysosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in lysosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in lysosome ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase MARCHF8
    Names
    E3 ubiquitin-protein ligase MARCH8
    RING-type E3 ubiquitin transferase MARCH8
    RING-type E3 ubiquitin transferase MARCHF8
    c-mir, cellular modulator of immune recognition
    cellular modulator of immune recognition
    membrane-associated RING finger protein 8
    membrane-associated RING-CH protein VIII
    membrane-associated ring finger (C3HC4) 8
    NP_001289312.1
    NP_001289313.1
    NP_001289314.1
    NP_001388597.1
    NP_001388598.1
    NP_001388599.1
    NP_001388600.1
    NP_001388601.1
    NP_001388602.1
    NP_082196.1
    XP_006506704.1
    XP_006506705.1
    XP_011239771.1
    XP_036008204.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001302383.2 → NP_001289312.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

      See identical proteins and their annotated locations for NP_001289312.1

      Status: REVIEWED

      Source sequence(s)
      AC123548
      Consensus CDS
      CCDS20451.1
      UniProtKB/Swiss-Prot
      Q9DBD2
      Related
      ENSMUSP00000078024.7, ENSMUST00000079012.13
      Conserved Domains (1) summary
      cd16698
      Location:75 → 126
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    2. NM_001302384.2 → NP_001289313.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

      See identical proteins and their annotated locations for NP_001289313.1

      Status: REVIEWED

      Source sequence(s)
      AC123548
      Consensus CDS
      CCDS20451.1
      UniProtKB/Swiss-Prot
      Q9DBD2
      Conserved Domains (1) summary
      cd16698
      Location:75 → 126
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    3. NM_001302385.2 → NP_001289314.1  E3 ubiquitin-protein ligase MARCHF8 isoform 2

      See identical proteins and their annotated locations for NP_001289314.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AC123548
      Consensus CDS
      CCDS85138.1
      UniProtKB/TrEMBL
      A0A0N4SVK0, F6THH9
      Related
      ENSMUSP00000145137.2, ENSMUST00000203193.3
      Conserved Domains (1) summary
      cd16698
      Location:36 → 87
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    4. NM_001401668.1 → NP_001388597.1  E3 ubiquitin-protein ligase MARCHF8 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AC123548
      UniProtKB/TrEMBL
      A0A0N4SV35
      Related
      ENSMUSP00000144936.2, ENSMUST00000123405.8

    5. NM_001401669.1 → NP_001388598.1  E3 ubiquitin-protein ligase MARCHF8 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AC123548

    6. NM_001401670.1 → NP_001388599.1  E3 ubiquitin-protein ligase MARCHF8 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AC123548
      UniProtKB/TrEMBL
      A0A0N4SV35

    7. NM_001401671.1 → NP_001388600.1  E3 ubiquitin-protein ligase MARCHF8 isoform 5

      Status: REVIEWED

      Source sequence(s)
      AC123548

    8. NM_001401672.1 → NP_001388601.1  E3 ubiquitin-protein ligase MARCHF8 isoform 6

      Status: REVIEWED

      Source sequence(s)
      AC123548
      Related
      ENSMUSP00000115510.3, ENSMUST00000135901.2

    9. NM_001401673.1 → NP_001388602.1  E3 ubiquitin-protein ligase MARCHF8 isoform 2

      Status: REVIEWED

      Source sequence(s)
      AC123548
      UniProtKB/TrEMBL
      A0A0N4SVK0

    10. NM_027920.6 → NP_082196.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

      See identical proteins and their annotated locations for NP_082196.1

      Status: REVIEWED

      Source sequence(s)
      AC123548
      Consensus CDS
      CCDS20451.1
      UniProtKB/Swiss-Prot
      Q9DBD2
      Related
      ENSMUSP00000098594.4, ENSMUST00000101032.10
      Conserved Domains (1) summary
      cd16698
      Location:75 → 126
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      116312546..116386501
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011241469.3 → XP_011239771.1  E3 ubiquitin-protein ligase MARCHF8 isoform X3

      See identical proteins and their annotated locations for XP_011239771.1

      Conserved Domains (1) summary
      cd16698
      Location:318 → 369
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    2. XM_006506641.5 → XP_006506704.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

      See identical proteins and their annotated locations for XP_006506704.1

      UniProtKB/TrEMBL
      A0A0N4SV35
      Conserved Domains (1) summary
      cd16698
      Location:357 → 408
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    3. XM_036152311.1 → XP_036008204.1  E3 ubiquitin-protein ligase MARCHF8 isoform X4

      UniProtKB/Swiss-Prot
      Q9DBD2
      Conserved Domains (1) summary
      cd16698
      Location:75 → 126
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

    4. XM_006506642.2 → XP_006506705.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

      See identical proteins and their annotated locations for XP_006506705.1

      UniProtKB/TrEMBL
      A0A0N4SV35
      Conserved Domains (1) summary
      cd16698
      Location:357 → 408
      RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9DBD2.1 GenPept UniProtKB/Swiss-Prot:Q9DBD2

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