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    Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 54283, updated on 2-Nov-2024

    Summary

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    Symbol
    Pfkfb4provided by RGD
    Full Name
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4provided by RGD
    Primary source
    RGD:3310
    See related
    EnsemblRapid:ENSRNOG00000020656 AllianceGenome:RGD:3310
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Enables 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to be active in cytosol. Orthologous to human PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in Kidney (RPKM 63.1), Testes (RPKM 43.7) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Pfkfb4 in Genome Data Viewer
    Location:
    8q32
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 8 NC_086026.1 (118522371..118565478)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 8 NC_051343.1 (109643558..109687006)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 8 NC_005107.4 (117733347..117776525)

    Chromosome 8 - NC_086026.1Genomic Context describing neighboring genes Neighboring gene collagen type VII alpha 1 chain Neighboring gene microRNA 711 Neighboring gene urocortin 2 Neighboring gene uncharacterized LOC100911671 Neighboring gene uncharacterized LOC134480237 Neighboring gene shisa family member 5

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of the H256A mutant of rat testis fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Fructose 6-phosphate in the active site leads to mechanisms for both mutant and wild type bisphosphatase activities. Yuen MH, et al. J Biol Chem, 1999 Jan 22. PMID 9890980
    2. The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies. Hasemann CA, et al. Structure, 1996 Sep 15. PMID 8805587
    3. Molecular cloning of the DNA and expression and characterization of rat testes fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase. Sakata J, et al. J Biol Chem, 1991 Aug 25. PMID 1651918
    4. Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes. Houddane A, et al. Cell Signal, 2017 Jun. PMID 28235572
    5. Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation. Gómez M, et al. Biochem Biophys Res Commun, 2009 Sep 18. PMID 19595670

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Increased PFKFB4 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
      Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
    2. The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
      Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables 6-phosphofructo-2-kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 6-phosphofructo-2-kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 6-phosphofructo-2-kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables fructose-2,6-bisphosphate 2-phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in carbohydrate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
    Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4-like
    6PF-2-K/Fru-2,6-P2ASE testis-type isozyme
    6PF-2-K/Fru-2,6-P2ase 4
    PFK/FBPase 4
    NP_062206.1
    XP_006243902.1
    XP_008764785.1
    XP_038937945.1
    XP_038937946.1
    XP_038937947.1
    XP_063122107.1
    XP_063122108.1
    XP_063122109.1
    XP_063122110.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001431583.1 → NP_001418512.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000008
      UniProtKB/Swiss-Prot
      P25114
      UniProtKB/TrEMBL
      A6I399

    2. NM_019333.2 → NP_062206.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

      See identical proteins and their annotated locations for NP_062206.1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000008
      UniProtKB/Swiss-Prot
      P25114
      UniProtKB/TrEMBL
      A6I399, B0ZTI0
      Related
      ENSRNOP00000028039.3, ENSRNOT00000028039.6
      Conserved Domains (2) summary
      pfam00300
      Location:252 → 438
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:30 → 249
      6PF2K; 6-phosphofructo-2-kinase

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086026.1 Reference GRCr8

      Range
      118522371..118565478
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006243840.5 → XP_006243902.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X2

      UniProtKB/TrEMBL
      A0A8L2R9R3, B0ZTI0
      Related
      ENSRNOP00000112787.1, ENSRNOT00000153908.1
      Conserved Domains (3) summary
      pfam00300
      Location:241 → 424
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:24 → 238
      6PF2K; 6-phosphofructo-2-kinase
      pfam13671
      Location:30 → 185
      AAA_33; AAA domain

    2. XM_008766563.4 → XP_008764785.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X1

      Conserved Domains (2) summary
      pfam00300
      Location:252 → 438
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:30 → 249
      6PF2K; 6-phosphofructo-2-kinase

    3. XM_039082018.2 → XP_038937946.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X6

      Conserved Domains (1) summary
      pfam01591
      Location:30 → 212
      6PF2K; 6-phosphofructo-2-kinase

    4. XM_039082019.2 → XP_038937947.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X7

      Conserved Domains (1) summary
      pfam01591
      Location:30 → 211
      6PF2K; 6-phosphofructo-2-kinase

    5. XM_063266037.1 → XP_063122107.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

    6. XM_063266038.1 → XP_063122108.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

    7. XM_063266039.1 → XP_063122109.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

      Related
      ENSRNOP00000074158.3, ENSRNOT00000090164.3

    8. XM_039082017.2 → XP_038937945.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X4

      Conserved Domains (2) summary
      pfam00300
      Location:61 → 247
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      cl17190
      Location:1 → 58
      NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

    9. XM_063266040.1 → XP_063122110.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X5

    RNA

    1. XR_010054022.1 RNA Sequence

    2. XR_010054021.1 RNA Sequence

    3. XR_005487907.2 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25114.3 GenPept UniProtKB/Swiss-Prot:P25114

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