mmp14a matrix metallopeptidase 14a (membrane-inserted) [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: mmp14aprovided by ZNC
Official Full Name: matrix metallopeptidase 14a (membrane-inserted)provided by ZNC
Primary source: ZFIN:ZDB-GENE-030901-1
See related: Ensembl:ENSDARG00000002235 AllianceGenome:ZFIN:ZDB-GENE-030901-1
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: cb386; sb:cb386; wu:fb72b09
Summary: Predicted to enable metalloendopeptidase activity and zinc ion binding activity. Acts upstream of with a positive effect on lamellipodium organization; regulation of cell migration; and regulation of extracellular matrix assembly. Acts upstream of or within several processes, including gastrulation; retinal ganglion cell axon guidance; and skeletal system development. Predicted to be located in extracellular matrix and membrane. Predicted to be active in extracellular space and plasma membrane. Is expressed in several structures, including axis; head; head mesenchyme; mesoderm; and musculature system. Human ortholog(s) of this gene implicated in Winchester syndrome; esophagus adenocarcinoma; and myocardial infarction. Orthologous to human MMP14 (matrix metallopeptidase 14). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 7

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	7	NC_007118.7 (24124298..24149631, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	7	NC_007118.6 (23853141..23878474, complement)

Chromosome 7 - NC_007118.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Based on our observations as well as previously published data, we hypothesize that MMP14's catalytic activity is the prime determinant of disease severity. Given the limitations of our in vitro assays in addressing the consequences of MMP14 dysfunction, we generated a novel mmp14a/b knockout zebrafish model
Title: Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype.
Data indicate Mmp14a-dependent retinal defects lead to microphthalmia; Mmp14b, on the contrary, does not appear to alter retinal neurogenesis.
Title: Matrix metalloproteinase 14 in the zebrafish: an eye on retinal and retinotectal development.
VANGL2 regulates the endocytosis and cell-surface availability of MMP14 in a manner that is dependent on focal adhesion kinase.
Title: VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration.
Data suggest that the MT1-MMP-PTK7 axis plays an important role in normal embryogenesis.
Title: The Wnt/planar cell polarity protein-tyrosine kinase-7 (PTK7) is a highly efficient proteolytic target of membrane type-1 matrix metalloproteinase: implications in cancer and embryogenesis.
Data show that Mmp14 is required for proper cell polarity underlying the directed migration of mesodermal cells during gastrulation, and identify a genetic interaction between mmp14 and non-canonical Wnt signaling.
Title: Membrane-type 1 matrix metalloproteinase regulates cell migration during zebrafish gastrulation: evidence for an interaction with non-canonical Wnt signaling.
MT-MMPalpha and beta were expressed through at least the first 72 h of development and this expression was targeted to the cell surface
Title: The expression of novel membrane-type matrix metalloproteinase isoforms is required for normal development of zebrafish embryos.

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General gene information

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Markers

G45483 (e-PCR)
- UniSTS:106285

fb72b09.x1 (e-PCR)
- UniSTS:203420

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	NAS Non-traceable Author Statement more info	PubMed
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within anterior/posterior axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cartilage development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell migration involved in gastrulation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in collagen catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within convergent extension	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within convergent extension involved in gastrulation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within convergent extension involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic cranial skeleton morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endochondral ossification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within establishment of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in extracellular matrix organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intramembranous ossification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_positive_effect lamellipodium organization	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_positive_effect lamellipodium organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural retina development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_positive_effect regulation of cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_positive_effect regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_positive_effect regulation of extracellular matrix assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_positive_effect regulation of extracellular matrix assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within retina layer formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in skeletal system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in zymogen activation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in extracellular matrix	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: matrix metalloproteinase-14a

Names: MT-MMPalpha; matrix metalloproteinase 14 (membrane-inserted) alpha; matrix metalloproteinase 14a (membrane-inserted); zMT-MMP-alpha; zMT-MMPalpha

NP_919397.1

EC 3.4.24.80

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_194416.1 → NP_919397.1 matrix metalloproteinase-14a precursor

See identical proteins and their annotated locations for NP_919397.1

Status: PROVISIONAL

Source sequence(s)

AY324196

UniProtKB/TrEMBL

Q7T2J2

Related

ENSDARP00000019511.6, ENSDART00000005884.8

Conserved Domains (5) summary

cd00094
Location:308 → 500

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

cd04278
Location:116 → 282

ZnMc_MMP; Zinc-dependent metalloprotease, matrix metalloproteinase (MMP) sub-family. MMPs are responsible for a great deal of pericellular proteolysis of extracellular matrix and cell surface molecules, playing crucial roles in morphogenesis, cell fate ...

pfam00413
Location:116 → 282

Peptidase_M10; Matrixin

pfam01471
Location:34 → 86

PG_binding_1; Putative peptidoglycan binding domain

pfam11857
Location:503 → 574

DUF3377; Domain of unknown function (DUF3377)