Dcr-2 Dicer-2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Dcr-2provided by FlyBase
Official Full Name: Dicer-2provided by FlyBase
Primary source: FLYBASE:FBgn0034246
Locus tag: Dmel_CG6493
See related: AllianceGenome:FB:FBgn0034246
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cg6493; CG6493; dcr; Dcr; dcr-2; DCR-2; dcr2; Dcr2; DCR2; dDcr2; dic2; dicer; Dicer; DICER; dicer-2; DICER-2; Dicer-II; dicer2; Dicer2; dmDcr-2; DmDcr-2; Dmel\CG6493
Summary: Enables ATP-dependent activity, acting on RNA; RNA binding activity; and bidentate ribonuclease III activity. Involved in several processes, including gene silencing by RNA; regulation of defense response; and response to virus. Located in cytoplasm and nucleus. Part of RISC complex and RISC-loading complex. Is expressed in adult head. Human ortholog(s) of this gene implicated in colorectal cancer; hepatocellular carcinoma; multinodular goiter; nephroma; and pleuropulmonary blastoma. Orthologous to human DICER1 (dicer 1, ribonuclease III). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 54C10-54C10; 2-83 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17574979..17581525, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (13462484..13469030, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Title: Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Title: Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.
Title: Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.
Transient kinetic studies of the antiviral Drosophila Dicer-2 reveal roles of ATP in self-nonself discrimination.
Title: Transient kinetic studies of the antiviral Drosophila Dicer-2 reveal roles of ATP in self-nonself discrimination.
TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity.
Title: TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity.
mutating Dicer-2 results in enhanced Zika virus load and increased susceptibility to Zika virus infection
Title: Dicer-2 Regulates Resistance and Maintains Homeostasis against Zika Virus Infection in Drosophila.
The results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation.
Title: Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation.
studies indicate that the 22 amino acids at the C terminus of Loqs-PD, including an FDF-like motif, directly interact with the Hel2 subdomain of Dicer-2's helicase domain. This interaction is RNA-independent, but we find that modulation of Dicer-2 cleavage also requires dsRNA binding by Loqs-PD.
Title: Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA.
this study used cryo-electron microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA.
Title: Dicer uses distinct modules for recognizing dsRNA termini.
Loqs-PD removes the inhibitory effect of inorganic phosphate on Dicer-2 processing of blunt-end hairpin RNAs, allowing siRNA production in the presence of inorganic phosphate.
Title: Loquacious-PD removes phosphate inhibition of Dicer-2 processing of hairpin RNAs into siRNAs.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent activity, acting on RNA	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables deoxyribonuclease I activity	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonuclease III activity	IBA Inferred from Biological aspect of Ancestor more info
enables ribonuclease III activity	IDA Inferred from Direct Assay more info	PubMed
enables ribonuclease III activity	IEA Inferred from Electronic Annotation more info
enables ribonuclease III activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in RNAi-mediated antiviral immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apoptotic DNA fragmentation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dosage compensation by hyperactivation of X chromosome	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dsRNA transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in global gene silencing by mRNA cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lncRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Toll signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of defense response to virus by host	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in pre-miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IDA Inferred from Direct Assay more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC-loading complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of RISC-loading complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Dicer-2

Names: CG6493-PA; CG6493-PB; Dcr-2-PA; Dcr-2-PB; Dicer2; dicer; dicer 2; dicer-2

NP_001286540.1

EC 3.1.21.1

EC 3.1.26.-

EC 3.1.26.3

NP_523778.2

EC 3.1.21.1

EC 3.1.26.-

EC 3.1.26.3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

17574979..17581525 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299611.1 → NP_001286540.1 Dicer-2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286540.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LFK8, Q2Q3W0

Conserved Domains (9) summary

smart00490
Location:438 → 495

HELICc; helicase superfamily c-terminal domain

smart00949
Location:842 → 1003

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

COG0571
Location:1432 → 1718

Rnc; dsRNA-specific ribonuclease [Transcription]

COG1111
Location:6 → 542

MPH1; ERCC4-related helicase [Replication, recombination and repair]

cd00046
Location:22 → 173

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd00593
Location:1447 → 1648

RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...

cd15903
Location:241 → 343

Dicer_PBD; Partner-binding domain of the endoribonuclease Dicer

pfam00035
Location:1652 → 1716

dsrm; Double-stranded RNA binding motif

pfam03368
Location:572 → 665

Dicer_dimer; Dicer dimerization domain
NM_079054.5 → NP_523778.2 Dicer-2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523778.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A1ZAW0, Q95YG3

UniProtKB/TrEMBL

Q2Q3W0

Related

FBpp0086061

Conserved Domains (9) summary

smart00490
Location:438 → 495

HELICc; helicase superfamily c-terminal domain

smart00949
Location:843 → 1004

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

COG0571
Location:1433 → 1719

Rnc; dsRNA-specific ribonuclease [Transcription]

COG1111
Location:6 → 542

MPH1; ERCC4-related helicase [Replication, recombination and repair]

cd00046
Location:22 → 173

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd00593
Location:1448 → 1649

RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...

cd15903
Location:241 → 343

Dicer_PBD; Partner-binding domain of the endoribonuclease Dicer

pfam00035
Location:1653 → 1717

dsrm; Double-stranded RNA binding motif

pfam03368
Location:572 → 665

Dicer_dimer; Dicer dimerization domain

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4LFK8		A0A0B4LFK8
A1ZAW0.1		A1ZAW0
Q2Q3V4		Q2Q3V4
Q2Q3V5		Q2Q3V5
Q2Q3V6		Q2Q3V6
Q2Q3V7		Q2Q3V7
Q2Q3V8		Q2Q3V8
Q2Q3V9		Q2Q3V9
Q2Q3W0		Q2Q3W0
Q2Q3W1		Q2Q3W1