Orc6 Origin recognition complex subunit 6 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Orc6provided by FlyBase
Official Full Name: Origin recognition complex subunit 6provided by FlyBase
Primary source: FLYBASE:FBgn0023180
Locus tag: Dmel_CG1584
See related: AllianceGenome:FB:FBgn0023180
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG1584; Dmel\CG1584; DmORC 6; DmORC6; ORC; orc6; ORC6; rDmORC
Summary: Enables GTPase activator activity and protein homodimerization activity. Involved in several processes, including DNA replication initiation; positive regulation of GTPase activity; and septin cytoskeleton organization. Located in plasma membrane and septin cytoskeleton. Part of nuclear origin of replication recognition complex. Is expressed in several structures, including embryonic brain; extended germ band embryo; and larval ventral nerve cord. Used to study Meier-Gorlin syndrome 3. Human ortholog(s) of this gene implicated in Meier-Gorlin syndrome 3. Orthologous to human ORC6 (origin recognition complex subunit 6). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 46B4-46B4; 2-61 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (9845458..9846485, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (5732963..5733990, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The interaction of the Drosophila ENY2 protein with the ORC complex subunits was investigated. It is found that ORC4 and ORC6 subunits directly interact with ENY2.
Title: Identification of the ORC Complex Subunits That Can Interact with the ENY2 Protein of Drosophila melanogaster.
In order to study the effects of Meier-Gorlin syndrome (MGS) mutation in an animal model system, MGS mutation in Orc6 was introduced and established Drosophila model of MGS.
Title: Drosophila model of Meier-Gorlin syndrome based on the mutation in a conserved C-Terminal domain of Orc6.
Drosophila Orc6 protein, the smallest subunit of the origin recognition complex (ORC), directly binds to septin complex and facilitates septin filament formation.
Title: Functional insight into the role of Orc6 in septin complex filament formation in Drosophila.
In a cross-species complementation experiment, the expression of human Orc6 in Drosophila Orc6 mutant cells rescued DNA replication, suggesting that this function of the protein is conserved among metazoans.
Title: Functional analysis of an Orc6 mutant in Drosophila.
These results show that the interaction of Orc6 with the septin complex is dependent on the coiled-coil domain of Pnut, and suggest an active role for Orc6 in septin complex function.
Title: Drosophila Orc6 facilitates GTPase activity and filament formation of the septin complex.
Drosophila ORC6 protein cytokinetic function resides in a domain distinct from its replication activity
Title: A cytokinetic function of Drosophila ORC6 protein resides in a domain distinct from its replication activity.
These studies provide a molecular analysis for the functional requirement of Orc6 in replicative functions of ORC in Drosophila and suggest that Orc6 may contribute to the sequence preferences of ORC in targeting to the origins.
Title: Role of the Orc6 protein in origin recognition complex-dependent DNA binding and replication in Drosophila melanogaster.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of nuclear origin of replication recognition complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear origin of replication recognition complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear origin of replication recognition complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of origin recognition complex	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in septin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: origin recognition complex subunit 6

Names: CG1584-PA; Orc6-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

9845458..9846485 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057971.6 → NP_477319.1 origin recognition complex subunit 6 [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477319.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V5C5, Q9Y1B2

Related

FBpp0087566

Conserved Domains (3) summary

cd11583
Location:97 → 185

Orc6_mid; Middle domain of the origin recognition complex subunit 6

cd16075
Location:187 → 242

ORC6_CTD; C-terminal domain of the eukaryotic origin recognition complex subunit ORC6

cl21687
Location:5 → 94

Orc6_mid; Middle domain of the origin recognition complex subunit 6