Adamts5 ADAM metallopeptidase with thrombospondin type 1 motif, 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Adamts5provided by RGD
Official Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 5provided by RGD
Primary source: RGD:735196
See related: EnsemblRapid:ENSRNOG00000057794 AllianceGenome:RGD:735196
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable several functions, including heparin binding activity; identical protein binding activity; and metalloendopeptidase activity. Predicted to be involved in several processes, including heart development; myoblast fusion; and negative regulation of cold-induced thermogenesis. Predicted to act upstream of or within defense response to bacterium. Predicted to be located in extracellular space. Predicted to be active in extracellular matrix. Used to study osteoarthritis. Biomarker of degenerative disc disease; mucopolysaccharidosis VI; and osteoarthritis. Human ortholog(s) of this gene implicated in osteoarthritis. Orthologous to human ADAMTS5 (ADAM metallopeptidase with thrombospondin type 1 motif 5). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 124.2), Heart (RPKM 91.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 11q11

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	11	NC_086029.1 (38486945..38533516, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	11	NC_051346.1 (25000627..25047205, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	11	NC_005110.4 (25411174..25456639, complement)

Chromosome 11 - NC_086029.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MiR-132-3p promotes rat mesenchymal stem cell chondrogenic differentiation, possibly mediated by targeting ADAMTS-5
Title: MiR-132-3p regulates ADAMTS-5 expression and promotes chondrogenic differentiation of rat mesenchymal stem cells.
OPG regulated the proliferation of chondrocytes via MEK/ERK signaling, and directly affected chondrocytes by influencing the expression profile of ADAMTS-5 and TIMP-4.
Title: Osteoprotegerin promotes the proliferation of chondrocytes and affects the expression of ADAMTS-5 and TIMP-4 through MEK/ERK signaling.
The aim of this study was to evaluate the effect of ADAMTS-5 knockdown on cartilage degradation.
Title: Protective effect of lentivirus-mediated siRNA targeting ADAMTS-5 on cartilage degradation in a rat model of osteoarthritis.
functional changes in the lumbar spine were at least partly associated with elevated ADAMTS-5, MMP-13, and TNF-alpha in a naturally occurring rat mucopolysaccharidosis VI model
Title: Structural, compositional, and biomechanical alterations of the lumbar spine in rats with mucopolysaccharidosis type VI (Maroteaux-Lamy syndrome).
Substate stiffness regulates the gene expression of Col1alpha1, Col2alpha1, aggrecan, MMP-3, MMp-13, and ADAMTS-5 in rat annular (intervertebral disc) cells.
Title: Substrate stiffness regulates apoptosis and the mRNA expression of extracellular matrix regulatory genes in the rat annular cells.
ADAMTS-5 mainly contributed to ECM metabolism in growth plate and condylar cartilage during growth. ADAMTS-1 and ADAMTS-4 may be involved in ECM turn over in articular cartilage.
Title: Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats.
Together these data demonstrate that inhibition of endogenous aggrecanases facilitates chondrocyte differentiation and chondrocyte-engineered cartilage formation in vitro.
Title: Lentivirus-mediated knockdown of aggrecanase-1 and -2 promotes chondrocyte-engineered cartilage formation in vitro.
This study suggests that dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes may be involved in both production and degradation of versican with secreting ADAMTS1, ADAMTS4 and ADAMTS5.
Title: Expression of versican and ADAMTS during rat tooth eruption.
Dysregulated genes expressed in rats with surgically-induced osteoarthritis included Mmp13, Adamts5, Ptges, Cxcr4, Ccl2, and Col2a1.
Title: Global analyses of gene expression in early experimental osteoarthritis.

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General gene information

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Markers

Adamts5 (e-PCR), detects polymorphism
- UniSTS:534410

Adamts5 (e-PCR), detects polymorphism
- UniSTS:532652

Adamts5 (e-PCR), detects polymorphism
- UniSTS:259033

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables extracellular matrix binding	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallopeptidase activity	ISO Inferred from Sequence Orthology more info
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aortic valve morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within defense response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in endocardial cushion morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in extracellular matrix disassembly	ISO Inferred from Sequence Orthology more info
involved_in extracellular matrix organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in myoblast fusion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cold-induced thermogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cold-induced thermogenesis	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in pulmonary valve morphogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in collagen-containing extracellular matrix	IEA Inferred from Electronic Annotation more info
is_active_in extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: A disintegrin and metalloproteinase with thrombospondin motifs 5

Names: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_198761.2 → NP_942056.2 A disintegrin and metalloproteinase with thrombospondin motifs 5 precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000011

UniProtKB/TrEMBL

A6JL67, G3V673, Q6TY19

Related

ENSRNOP00000068781.1, ENSRNOT00000085444.3

Conserved Domains (6) summary

smart00209
Location:567 → 619

TSP1; Thrombospondin type 1 repeats

cd04273
Location:268 → 470

ZnMc_ADAMTS_like; Zinc-dependent metalloprotease, ADAMTS_like subgroup. ADAMs (A Disintegrin And Metalloprotease) are glycoproteins, which play roles in cell signaling, cell fusion, and cell-cell interactions. This particular subfamily represents domain architectures that ...

pfam05986
Location:729 → 849

ADAM_spacer1; ADAM-TS Spacer 1

pfam01562
Location:61 → 181

Pep_M12B_propep; Reprolysin family propeptide

pfam17771
Location:484 → 551

ADAM_CR_2; ADAM cysteine-rich domain

pfam19030
Location:876 → 927

TSP1_ADAMTS; Thrombospondin type 1 domain