Cadps calcium dependent secretion activator [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Cadpsprovided by RGD
Full Name: calcium dependent secretion activatorprovided by RGD
Primary source: RGD:708573
See related: EnsemblRapid:ENSRNOG00000008570 AllianceGenome:RGD:708573
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Caps; Caps1; Caps2
Summary: Enables calcium ion binding activity and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in positive regulation of calcium ion-dependent exocytosis. Located in dense core granule. Is active in glutamatergic synapse and presynapse. Is extrinsic component of neuronal dense core vesicle membrane. Orthologous to human CADPS (calcium dependent secretion activator). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 424.1), Adrenal (RPKM 89.7) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 15p15

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (14719078..15173218)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (12289803..12742786)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (15275489..15690575)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Down-regulation of habenular calcium-dependent secretion activator 2 induces despair-like behavior.
Title: Down-regulation of habenular calcium-dependent secretion activator 2 induces despair-like behavior.
The unique tandem C2-PH domain of CAPS may serve as a PI(4,5)P2-triggered switch for dimerization. CAPS dimerization may be coupled to oligomeric SNARE complex assembly for vesicle docking and priming.
Title: The Vesicle Priming Factor CAPS Functions as a Homodimer via C2 Domain Interactions to Promote Regulated Vesicle Exocytosis.
results indicate that dense-core vesicles carry CAPS to sites of exocytosis, where CAPS promotes vesicle docking and fusion competence, probably by initiating SNARE complex assembly.
Title: Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.
Transport vesicle fusion into PIP2-rich membrane domains is facilitated by sequential PIP2-dependent activation of CAPS and PIP2-dependent recruitment of Munc13.
Title: CAPS and Munc13 utilize distinct PIP2-linked mechanisms to promote vesicle exocytosis.
CAPS1 binds to the full-length of cytoplasmic syntaxin-1 with preference to its "open" conformation, whereas Munc13-1 binds to the first 80 N-terminal residues of syntaxin-1.
Title: Calcium-dependent activator protein for secretion 1 (CAPS1) binds to syntaxin-1 in a distinct mode from Munc13-1.
The results show that Munc13 homology domain-1 is a SNARE-binding domain and that SNARE protein binding is essential for CAPS function in dense-core vesicle exocytosis.
Title: Munc13 homology domain-1 in CAPS/UNC31 mediates SNARE binding required for priming vesicle exocytosis.
Results suggest that SNARE complex formation promoted by CAPS may be mediated by direct interactions of CAPS with each of the three SNARE proteins required.
Title: Novel interactions of CAPS (Ca2+-dependent activator protein for secretion) with the three neuronal SNARE proteins required for vesicle fusion.
a functional role for CAPS1 in regulating DCV trafficking in the trans-Golgi network; this activity occurs via protein-protein interaction with ARF4/ARF5 in a GDP-dependent manner.
Title: Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Data suggest that CAPS functions in priming vesicle exocytosis by coupling membrane binding to SNARE complexes. assembly.
Title: Phosphatidylinositol 4,5-bisphosphate regulation of SNARE function in membrane fusion mediated by CAPS.
CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation
Title: CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU048310 (e-PCR)
- UniSTS:109662

D15Rat159 (e-PCR), detects polymorphism
- UniSTS:118406

D15Rat137 (e-PCR), detects polymorphism
- UniSTS:226697

AW531782 (e-PCR)
- UniSTS:260513

RH135013 (e-PCR)
- UniSTS:218290

RH138000 (e-PCR)
- UniSTS:220087

RH136728 (e-PCR)
- UniSTS:218817

BF388496 (e-PCR)
- UniSTS:244962

BF388503 (e-PCR)
- UniSTS:244971

RH134048 (e-PCR)
- UniSTS:217330

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in catecholamine secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within catecholamine secretion	ISO Inferred from Sequence Orthology more info
involved_in dense core granule exocytosis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
NOT acts_upstream_of_or_within exocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of calcium ion-dependent exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic dense core vesicle exocytosis	IEA Inferred from Electronic Annotation more info
involved_in presynaptic dense core vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulated exocytosis	TAS Traceable Author Statement more info	PubMed
involved_in synaptic vesicle exocytosis	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle priming	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within synaptic vesicle priming	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle priming	ISO Inferred from Sequence Orthology more info
involved_in vesicle organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within vesicle organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in dense core granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of neuronal dense core vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: calcium-dependent secretion activator 1

Names: CAPS-1; Ca++-dependent secretion activator; Ca2+ dependent secretion activator; Ca2+-dependent activator protein; Ca<2+-dependent activator protein for secretion; calcium-dependent activator protein for secretion 1; calcium-dependent secretion activator 1-like; rCAPS

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013219.2 → NP_037351.2 calcium-dependent secretion activator 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000015

UniProtKB/TrEMBL

A0A8I5Y0W4, A6K086, F1LLX6

Related

ENSRNOP00000076873.2, ENSRNOT00000094744.2

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 925

DUF1041; Domain of Unknown Function (DUF1041)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086033.1 Reference GRCr8

Range

14719078..15173218

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039093071.2 → XP_038948999.1 calcium-dependent secretion activator 1 isoform X15

UniProtKB/TrEMBL

F1LLX6

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:887 → 978

DUF1041; Domain of Unknown Function (DUF1041)
XM_063274046.1 → XP_063130116.1 calcium-dependent secretion activator 1 isoform X1

UniProtKB/TrEMBL

A0A8I6AGZ8

Related

ENSRNOP00000095354.2, ENSRNOT00000099157.2
XM_039093064.2 → XP_038948992.1 calcium-dependent secretion activator 1 isoform X9

UniProtKB/TrEMBL

F1LLX6

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 961

DUF1041; Domain of Unknown Function (DUF1041)
XM_063274049.1 → XP_063130119.1 calcium-dependent secretion activator 1 isoform X3

UniProtKB/TrEMBL

A0A8I6AGZ8

Related

ENSRNOP00000092113.2, ENSRNOT00000098925.2
XM_063274052.1 → XP_063130122.1 calcium-dependent secretion activator 1 isoform X10

UniProtKB/TrEMBL

F1LLX6
XM_063274048.1 → XP_063130118.1 calcium-dependent secretion activator 1 isoform X2

UniProtKB/TrEMBL

A0A8I6ATV6
XM_039093050.2 → XP_038948978.1 calcium-dependent secretion activator 1 isoform X4

UniProtKB/TrEMBL

A0A8I6A5K7, A0A8I6ATV6

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 948

DUF1041; Domain of Unknown Function (DUF1041)
XM_039093067.2 → XP_038948995.1 calcium-dependent secretion activator 1 isoform X11

UniProtKB/TrEMBL

F1LLX6

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 948

DUF1041; Domain of Unknown Function (DUF1041)
XM_039093055.2 → XP_038948983.1 calcium-dependent secretion activator 1 isoform X5

UniProtKB/TrEMBL

A0A8I6AGZ8

Related

ENSRNOP00000093742.2, ENSRNOT00000105737.2

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 938

DUF1041; Domain of Unknown Function (DUF1041)
XM_063274050.1 → XP_063130120.1 calcium-dependent secretion activator 1 isoform X7

UniProtKB/TrEMBL

A0A8I6AGZ8
XM_063274054.1 → XP_063130124.1 calcium-dependent secretion activator 1 isoform X12

UniProtKB/TrEMBL

F1LLX6
XM_039093060.2 → XP_038948988.1 calcium-dependent secretion activator 1 isoform X6

UniProtKB/TrEMBL

A0A8I6AGZ8

Conserved Domains (2) summary

cd01234
Location:513 → 632

PH_CADPS; Ca2+-dependent activator protein (also called CAPS) Pleckstrin homology (PH) domain

pfam06292
Location:834 → 932

DUF1041; Domain of Unknown Function (DUF1041)
XM_063274051.1 → XP_063130121.1 calcium-dependent secretion activator 1 isoform X8

UniProtKB/TrEMBL

A0A8I6AGZ8, A0A8I6AI45
XM_063274055.1 → XP_063130125.1 calcium-dependent secretion activator 1 isoform X13
XM_063274056.1 → XP_063130126.1 calcium-dependent secretion activator 1 isoform X14