Pfkl phosphofructokinase, liver type [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pfklprovided by RGD
Official Full Name: phosphofructokinase, liver typeprovided by RGD
Primary source: RGD:3311
See related: EnsemblRapid:ENSRNOG00000001214 AllianceGenome:RGD:3311
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PFK-L; ATP-PFK
Summary: Enables several functions, including 6-phosphofructokinase activity; anion binding activity; and identical protein binding activity. Involved in fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; and glycolytic process. Predicted to be located in cytosol. Predicted to be part of 6-phosphofructokinase complex. Predicted to be active in membrane. Orthologous to human PFKL (phosphofructokinase, liver type). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Adrenal (RPKM 188.6), Kidney (RPKM 158.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 20p12

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (10663907..10685967)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (10664285..10686324)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (11393860..11415889)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

kinetic model of liver phosphofructokinase from rat
Title: Critical switch of the metabolic fluxes by phosphofructo-2-kinase:fructose-2,6-bisphosphatase. A kinetic model.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH94598 (e-PCR)
- UniSTS:119846

Pfkl (e-PCR), detects polymorphism
- UniSTS:259128

RH130425 (e-PCR)
- UniSTS:213709

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructokinase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to 6-phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables 6-phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables 6-phosphofructokinase activity	TAS Traceable Author Statement more info	PubMed
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables fructose binding	ISO Inferred from Sequence Orthology more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	TAS Traceable Author Statement more info	PubMed
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-6-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables fructose-6-phosphate binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monosaccharide binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within carbohydrate phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 1,6-bisphosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within fructose 6-phosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fructose 6-phosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glycolytic process through fructose-6-phosphate	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process through fructose-6-phosphate	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to glucose	ISO Inferred from Sequence Orthology more info
involved_in response to glucose	ISO Inferred from Sequence Orthology more info
involved_in response to glucose	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ATP-dependent 6-phosphofructokinase, liver type

Names: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; PFK-B; phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver, B-type; phosphohexokinase

NP_037322.1

EC 2.7.1.11

XP_008771020.1

EC 2.7.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013190.4 → NP_037322.1 ATP-dependent 6-phosphofructokinase, liver type

See identical proteins and their annotated locations for NP_037322.1

Status: PROVISIONAL

Source sequence(s)

X58865

UniProtKB/Swiss-Prot

P30835

UniProtKB/TrEMBL

Q6P783

Related

ENSRNOP00000100786.1, ENSRNOT00000147989.1

Conserved Domains (1) summary

cd00764
Location:13 → 773

Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086038.1 Reference GRCr8

Range

10663907..10685967

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008772798.4 → XP_008771020.1 ATP-dependent 6-phosphofructokinase, liver type isoform X1

UniProtKB/TrEMBL

Q6P783

Related

ENSRNOP00000001625.4, ENSRNOT00000001625.7

Conserved Domains (2) summary

cd00764
Location:13 → 796

Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

TIGR02478
Location:16 → 778

6PF1K_euk; 6-phosphofructokinase, eukaryotic type