Chrna5 cholinergic receptor nicotinic alpha 5 subunit [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chrna5provided by RGD
Official Full Name: cholinergic receptor nicotinic alpha 5 subunitprovided by RGD
Primary source: RGD:2347
See related: EnsemblRapid:ENSRNOG00000013610 AllianceGenome:RGD:2347
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables acetylcholine binding activity and acetylcholine-gated monoatomic cation-selective channel activity. Involved in several processes, including behavioral response to nicotine; cellular response to nicotine; and detection of mechanical stimulus involved in sensory perception of pain. Located in dendrite and neuronal cell body. Part of acetylcholine-gated channel complex. Human ortholog(s) of this gene implicated in chronic obstructive pulmonary disease; cocaine abuse; esophagus squamous cell carcinoma; nicotine dependence; and respiratory system cancer (multiple). Orthologous to human CHRNA5 (cholinergic receptor nicotinic alpha 5 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 18.6), Adrenal (RPKM 6.2) and 4 other tissues See more
Orthologs: human mouse all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 8q24

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (64265879..64294233)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (55369794..55398526)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (59561817..59590172)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rats expressing the alpha5SNP consume more alcohol, and exhibit increased relapse to alcohol seeking after abstinence.
Title: Profound alteration in reward processing due to a human polymorphism in CHRNA5: a role in alcohol dependence and feeding behavior.
The alpha5SNP polymorphism is a major risk factor for nicotine intake at high doses and for relapse to nicotine seeking in rats, a dual effect that reflects the human condition. The results also suggest an important role for the IPN in the higher relapse to nicotine seeking observed in alpha5SNP rats.
Title: A Human Polymorphism in CHRNA5 Is Linked to Relapse to Nicotine Seeking in Transgenic Rats.
This study provide evidence that through its contribution to cholinergic modulation of cholinergic modulation of neurons in the PFC, the alpha5 nAChR plays a specific role in the recovery of attention task performance following distraction.
Title: α5 nAChR modulation of the prefrontal cortex makes attention resilient.
We found low levels of alpha5-containing receptors in rats
Title: Subunit composition of α5-containing nicotinic receptors in the rodent habenula.
increased nicotine intake with a null mutation in Chrna5
Title: Habenular α5 nicotinic receptor subunit signalling controls nicotine intake.
alpha5 is an influential modulator of alpha4+beta2 nicotinic acetylcholine receptor assembly and stabilizes their expression in response to fluctuations in external conditions.
Title: Nicotinic receptor subunit alpha5 modifies assembly, up-regulation, and response to pro-inflammatory cytokines.
Increased expression of the alpha 5 nAChR subunit following spinal nerve ligation contributes to spinal cAMP response element-binding protein phosphorylation and the maintenance of mechanical allodynia.
Title: Knock down of the alpha 5 nicotinic acetylcholine receptor in spinal nerve-ligated rats alleviates mechanical allodynia.
Similar to other nAChR subunits, there is transient expression of alpha5 mRNA during cortical and hippocampal development.
Title: Expression of alpha5 nicotinic acetylcholine receptor subunit mRNA during hippocampal and cortical development.
Immunocytochemistry revealed most chromaffin cells positive for alpha5 proteins.
Title: Molecular biology and electrophysiology of neuronal nicotinic receptors of rat chromaffin cells.
Calcium-imaging and double-labeling immunofluorescence with alpha-subunit-specific antibodies, in combination with markers for nociceptive neurons are examined.
Title: Nicotinic acetylcholine receptors containing subunits alpha3 and alpha5 in rat nociceptive dorsal root ganglion neurons.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

Go to the top of the page Help

Markers

ha2920 (e-PCR)
- UniSTS:515612

Chrna5 (e-PCR), detects polymorphism
- UniSTS:261414

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acetylcholine binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in behavioral response to nicotine	IEA Inferred from Electronic Annotation more info
involved_in behavioral response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in detection of mechanical stimulus involved in sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
involved_in hippocampus development	IEP Inferred from Expression Pattern more info	PubMed
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in presynaptic modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in presynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuronal action potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor activity	ISO Inferred from Sequence Orthology more info
involved_in response to nicotine	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, cholinergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, cholinergic	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IDA Inferred from Direct Assay more info	PubMed
part_of acetylcholine-gated channel complex	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
located_in cholinergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in cholinergic synapse	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dopaminergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in dopaminergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
located_in presynapse	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

Go to the top of the page Help

Preferred Names: neuronal acetylcholine receptor subunit alpha-5

Names: acetylcholine receptor alpha 5; cholinergic receptor, nicotinic, alpha 5 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 5

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017078.2 → NP_058774.2 neuronal acetylcholine receptor subunit alpha-5

See identical proteins and their annotated locations for NP_058774.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

A0A8J8XM73, A6J4N6, G3V9X4, Q6PW50

Related

ENSRNOP00000062227.3, ENSRNOT00000065771.5

Conserved Domains (3) summary

TIGR00860
Location:41 → 448

LIC; Cation transporter family protein

pfam02931
Location:47 → 250

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:257 → 446

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region