Pgk2 phosphoglycerate kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pgk2provided by MGI
Official Full Name: phosphoglycerate kinase 2provided by MGI
Primary source: MGI:MGI:97563
See related: Ensembl:ENSMUSG00000031233 AllianceGenome:MGI:97563
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pgk-2; Tcp-2
Summary: Enables phosphoglycerate kinase activity. Acts upstream of or within flagellated sperm motility and glycolytic process. Located in sperm fibrous sheath. Is expressed in germ cell of testis and testis. Orthologous to human PGK2 (phosphoglycerate kinase 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

Location:: 17 B2; 17 19.44 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (40517909..40519500, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (40207018..40208609, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PGK2 is expressed differently in the testes of mouse and pigs according to developmental stage.
Title: Species-specific expression of phosphoglycerate kinase 2 (PGK2) in the developing porcine testis.
These results identify multiple signaling and metabolic defects that are likely contributors to male infertility and the absence of progressive sperm motility seen in mice lacking GAPDHS.
Title: Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes.
Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis.
Title: Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.
not required for the completion of spermatogenesis, but essential for sperm motility and male fertility
Title: Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.
t-KH-type splicing regulatory protein binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts.
Title: A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells.
Pgk2 crystal structures have been solved; the apoenzyme is in the "open" form; that is, the N-terminal domain that can bind 3-phosphoglycerate and the C-terminal domain that binds ATP are too far apart for the substrates to interact.
Title: X-ray analysis of phosphoglycerate kinase 2, a sperm-specific isoform from Mus musculus.
Data suggest that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene.
Title: In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis.
PTBP2 is a trans-acting factor that helps to stabilize Pgk2 mRNA in male mouse germ cells.
Title: Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3'UTR.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

mPgk2 (e-PCR)
- UniSTS:514588

Pgk2 (e-PCR), detects polymorphism
- UniSTS:526976

Pgk2 (e-PCR), detects polymorphism
- UniSTS:265423

Nif3l1 (e-PCR), detects polymorphism
- UniSTS:207061

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ADP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables phosphoglycerate kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoglycerate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoglycerate kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphoglycerate kinase activity	ISO Inferred from Sequence Orthology more info
enables phosphoglycerate kinase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glycolytic process	IC Inferred by Curator more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cilium	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in sperm fibrous sheath	IBA Inferred from Biological aspect of Ancestor more info
located_in sperm fibrous sheath	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphoglycerate kinase 2

Names: phosphoglycerate kinase, testis specific

NP_112467.2

EC 2.7.2.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.