Epha3 Eph receptor A3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Epha3provided by MGI
Official Full Name: Eph receptor A3provided by MGI
Primary source: MGI:MGI:99612
See related: Ensembl:ENSMUSG00000052504 AllianceGenome:MGI:99612
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EK4; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4
Summary: Enables ephrin receptor activity and growth factor binding activity. Involved in several processes, including axonal fasciculation; ephrin receptor signaling pathway; and synaptic membrane adhesion. Acts upstream of or within cellular response to follicle-stimulating hormone stimulus; negative regulation of neuron projection development; and regulation of cell migration. Is active in GABA-ergic synapse and presynapse. Is expressed in several structures, including alimentary system; embryo mesenchyme; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human EPHA3 (EPH receptor A3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in CNS E11.5 (RPKM 8.7), CNS E14 (RPKM 8.4) and 11 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 C1.3; 16 36.49 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (63363895..63684538, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (63543532..63864175, complement)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

EPHA3 enhances macrophage autophagy and apoptosis by disrupting the mTOR signaling pathway in mice with endometriosis.
Title: EPHA3 enhances macrophage autophagy and apoptosis by disrupting the mTOR signaling pathway in mice with endometriosis.
In conclusion, PS1/gamma-secretase-dependent EphA3 cleavage mediates axon growth by regulating filament assembly through RhoA signaling and non-muscle myosin IIA (NMIIA), suggesting opposite roles of EphA3 on inhibiting (ligand-dependent) and promoting (receptor processing) axon growth in developing neurons.
Title: Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA.
Although EPHA3 was reported to be one of the most frequently mutated genes in colorectal tumors, our studies using inducible isogenic cell line systems, mouse models and large human tumor collections, did not reveal a major role of this EPH receptor on proliferation/motility/invasion of cancer cells, tumor initiation/progression/metastasis in mouse models or survival of colorectal cancer patients.
Title: Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer.
study delineates a mechanism in which NCAM promotes ephrin-A5-dependent clustering of EphA3 through interaction of the NCAM Ig2 domain and the EphA3 CRD, stimulating EphA3 autophosphorylation and RhoA signaling necessary for growth cone repulsion in GABAergic interneurons in vitro, which may extend to remodeling of axonal terminals of interneurons in vivo.
Title: The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse.
Ephrin-A3 has a role in promoting and maintaining slow muscle fiber identity during postnatal development and reinnervation
Title: Ephrin-A3 promotes and maintains slow muscle fiber identity during postnatal development and reinnervation.
the physiological role of the putative lung cancer tumor suppressor EPH receptor A3, is reported.
Title: The putative tumor suppressor gene EphA3 fails to demonstrate a crucial role in murine lung tumorigenesis or morphogenesis.
Mutations in EPHA family genes may cause cleft lip/palate with functional redundancy between family members perhaps at play.
Title: Embryonic expression of EphA receptor genes in mice supports their candidacy for involvement in cleft lip and palate.
ephrinA5/EphA3 triggers proteolysis of the neural cell adhesion molecule (NCAM) by the metalloprotease a disintegrin and metalloprotease (ADAM)10 to promote growth cone collapse in neurons from mouse neocortex.
Title: EphrinA/EphA-induced ectodomain shedding of neural cell adhesion molecule regulates growth cone repulsion through ADAM10 metalloprotease.
EphA3 receptor localized only in neuronal cells of the hippocampus was enhanced without transcriptional regulation during synaptic plasticity through activation of the nicotinic acetylcholine receptor.
Title: The expression changes of EphA3 receptor during synaptic plasticity in mouse hippocampus through activation of nicotinic acetylcholine receptor.
Data show that a number of Eph receptors and ephrins were expressed in hematopoietic stem cells.
Title: Activation of ephrin A proteins influences hematopoietic stem cell adhesion and trafficking patterns.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Epha3 (e-PCR), detects polymorphism
- UniSTS:495881

Epha3 (e-PCR), detects polymorphism
- UniSTS:498468

M68513 (e-PCR)
- UniSTS:160579

NoName (e-PCR)
- UniSTS:224333

NoName (e-PCR)
- UniSTS:548479

AW492086 (e-PCR)
- UniSTS:180927

UniSTS:498373 (e-PCR)
- UniSTS:498373

Epha3 (e-PCR), detects polymorphism
- UniSTS:498373

Epha3 (e-PCR), detects polymorphism
- UniSTS:498374

Epha3 (e-PCR), detects polymorphism
- UniSTS:498375

Epha3 (e-PCR), detects polymorphism
- UniSTS:527334

Epha3 (e-PCR), detects polymorphism
- UniSTS:266986

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables GPI-linked ephrin receptor activity	ISO Inferred from Sequence Orthology more info
enables GPI-linked ephrin receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables ephrin receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables ephrin receptor activity	ISO Inferred from Sequence Orthology more info
enables growth factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane-ephrin receptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to follicle-stimulating hormone stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to retinoic acid	ISO Inferred from Sequence Orthology more info
involved_in endocardial cushion development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ephrin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in ephrin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in ephrin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in fasciculation of motor neuron axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fasciculation of sensory neuron axon	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in motor neuron axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of endocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of neuron projection development	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of epithelial to mesenchymal transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of focal adhesion assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of microtubule cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of microtubule cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in synaptic membrane adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic membrane adhesion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in GABA-ergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ephrin type-A receptor 3

Names: EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1

NP_001349381.1

EC 2.7.10.1

NP_034270.1

EC 2.7.10.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362452.1 → NP_001349381.1 ephrin type-A receptor 3 isoform 2 precursor

Status: VALIDATED

Source sequence(s)

AK089119, AW491216, BC093483, BC145349

Consensus CDS

CCDS88948.1

UniProtKB/TrEMBL

B7ZNN5, Q8C3U1

Related

ENSMUSP00000155946.2, ENSMUST00000232049.2

Conserved Domains (6) summary

cd09544
Location:912 → 974

SAM_EPH-A3; SAM domain of EPH-A3 subfamily of tyrosine kinase receptors

cd10481
Location:29 → 201

EphR_LBD_A3; Ligand Binding Domain of Ephrin type-A Receptor 3

cd00063
Location:437 → 527

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:616 → 882

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam00041
Location:327 → 417

fn3; Fibronectin type III domain

pfam14575
Location:564 → 616

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain
NM_010140.4 → NP_034270.1 ephrin type-A receptor 3 isoform 1 precursor

See identical proteins and their annotated locations for NP_034270.1

Status: VALIDATED

Source sequence(s)

AC154568, AC154844, AC155275

Consensus CDS

CCDS28264.1

UniProtKB/TrEMBL

Q8BRB1, Q8C3U1

Related

ENSMUSP00000066554.7, ENSMUST00000064405.8

Conserved Domains (8) summary

cd09544
Location:913 → 975

SAM_EPH-A3; SAM domain of EPH-A3 subfamily of tyrosine kinase receptors

smart00454
Location:909 → 976

SAM; Sterile alpha motif

cd10481
Location:29 → 201

EphR_LBD_A3; Ligand Binding Domain of Ephrin type-A Receptor 3

cd00063
Location:437 → 528

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd05066
Location:617 → 883

PTKc_EphR_A; Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors

pfam00041
Location:327 → 417

fn3; Fibronectin type III domain

pfam07714
Location:622 → 879

Pkinase_Tyr; Protein tyrosine kinase

pfam14575
Location:566 → 617

EphA2_TM; Ephrin type-A receptor 2 transmembrane domain