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    crp DNA-binding transcriptional dual regulator CRP [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947867, updated on 19-Sep-2024

    Summary

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    Official Symbol
    crp
    Official Full Name
    DNA-binding transcriptional dual regulator CRP
    Primary source
    ECOCYC:EG10164
    Locus tag
    b3357
    See related
    ASAP:ABE-0010970
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cap; csm; ECK3345; gurB
    Summary
    [More information is available at EcoCyc: EG10164].
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    Genomic context

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    Sequence:
    NC_000913.3 (3486120..3486752)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative phosphoribulokinase Neighboring gene OsmC family protein YhfA Neighboring gene putative transporter YhfK Neighboring gene N-acetylornithine aminotransferase/N-succinyldiaminopimelate aminotransferase Neighboring gene protein YrfJ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase. Niu W, et al. Cell, 1996 Dec 13. PMID 8978616, Free PMC Article
    2. A C-helix residue, Arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Youn H, et al. J Biol Chem, 2007 Feb 9. PMID 17158453
    3. Mode of selectivity in cyclic AMP receptor protein-dependent promoters in Escherichia coli. Pyles EA, et al. Biochemistry, 1996 Jan 30. PMID 8573570
    4. CRP subunit association and hinge conformation changes in response to cAMP binding: analysis of C-helix cysteine-substituted CRP. Tomlinson SR, et al. Biochemistry, 2006 Nov 14. PMID 17087497
    5. Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters. Williams RM, et al. Nucleic Acids Res, 1996 Mar 15. PMID 8604346, Free PMC Article

    See all (325) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Regulation of Aerobic Succinate Transporter dctA of E. coli by cAMP-CRP, DcuS-DcuR, and EIIAGlc: Succinate as a Carbon Substrate and Signaling Molecule.
      Title: Regulation of Aerobic Succinate Transporter dctA of E. coli by cAMP-CRP, DcuS-DcuR, and EIIAGlc: Succinate as a Carbon Substrate and Signaling Molecule.
    2. Restoring Global Gene Regulation through Experimental Evolution Uncovers a NAP (Nucleoid-Associated Protein)-Like Behavior of Crp/Cap.
      Title: Restoring Global Gene Regulation through Experimental Evolution Uncovers a NAP (Nucleoid-Associated Protein)-Like Behavior of Crp/Cap.
    3. Temporal evolution of master regulator Crp identifies pyrimidines as catabolite modulator factors.
      Title: Temporal evolution of master regulator Crp identifies pyrimidines as catabolite modulator factors.
    4. Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage.
      Title: Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage.
    5. The CRP subunits are energetically asymmetricand that mutations exert their effects differentially between the two subunits through networks of long-range communication.
      Title: Differential modulation of energy landscapes of cyclic AMP receptor protein (CRP) as a regulatory mechanism for class II CRP-dependent promoters.
    6. Hydrogen sulfide (H2S) is produced by Escherichia coli cells growing in a synthetic minimal medium containing thiosulfate as a sole inorganic sulfur source, but not in a medium only containing sulfate. E. coli generated H2S in a DNA-binding transcriptional dual regulator CRP-dependent manner as a response to glucose starvation.
      Title: Generation of hydrogen sulfide from sulfur assimilation in Escherichia coli.
    7. Escherichia coli deletion mutants lacking cAMP-receptor protein (CRP) maintains colony-forming activities even after a long period of starvation, while wild-type cells lost most of this activity. The lack of cAMP-CRP effectively retains high colony-forming activities, indicating that cAMP-CRP acts as a positive regulator necessary for the induction of the viable but non-culturable state in E. coli.
      Title: cAMP-CRP acts as a key regulator for the viable but non-culturable state in Escherichia coli.
    8. Data show that DNA-binding transcriptional dual regulators Cra and CRP are most active on poor carbon sources.
      Title: Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP.
    9. Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes.
      Title: Escherichia coli OxyS RNA triggers cephalothin resistance by modulating the expression of CRP-associated genes.
    10. Study compares the ability of two statistical mechanical models of protein conformational change-to characterize the multi-step activation mechanism of the broadly acting CRP. These models have the predictive power to determine how combinations of mutations will interact, how double mutants will behave, and how each construct would regulate gene expression.
      Title: Theoretical analysis of inducer and operator binding for cyclic-AMP receptor protein mutants.
    Submit: New GeneRIF Correction See all GeneRIFs (66)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding, bending IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cAMP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables minor groove of adenine-thymine-rich DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated transcription IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within carbon catabolite repression of transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator CRP

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3486120..3486752
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417816.1 DNA-binding transcriptional dual regulator CRP [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417816.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AP45, A0A8S0FJE1
      Conserved Domains (1) summary
      PRK11753
      Location:1 → 210
      PRK11753; cAMP-activated global transcriptional regulator CRP
    Nucleotide Protein
    Heading Accession and Version

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