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mnmE 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948222, updated on 19-Sep-2024

Summary

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Official Symbol
mnmE
Official Full Name
5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit
Primary source
ECOCYC:EG10997
Locus tag
b3706
See related
ASAP:ABE-0012123
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3699; thdF; trmE
Summary
GTPase activity is essential for modification function. [More information is available at EcoGene: EG10997]. MnmE is required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA . [More information is available at EcoCyc: EG10997].
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Genomic context

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Sequence:
NC_000913.3 (3886828..3888192)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene RNase P protein component Neighboring gene membrane protein insertion efficiency factor Neighboring gene membrane protein insertase YidC Neighboring gene tnaAB operon leader peptide Neighboring gene tryptophanase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Effects of mutagenesis in the switch I region and conserved arginines of Escherichia coli MnmE protein, a GTPase involved in tRNA modification. Martínez-Vicente M, et al. J Biol Chem, 2005 Sep 2. PMID 15983041
  2. Molecular cloning and sequence of the thdF gene, which is involved in thiophene and furan oxidation by Escherichia coli. Alam KY, et al. J Bacteriol, 1991 Oct. PMID 1917835, Free PMC Article
  3. Regulation of the thdF gene, which is involved in thiophene oxidation by Escherichia coli K-12. Zabel MD, et al. Microbios, 2000. PMID 10738982
  4. The GTPase activity and C-terminal cysteine of the Escherichia coli MnmE protein are essential for its tRNA modifying function. Yim L, et al. J Biol Chem, 2003 Aug 1. PMID 12730230
  5. Backbone 1H, 13C and 15N resonance assignments for the 18.7 kDa GTPase domain of Escherichia coli MnmE protein. Monleón D, et al. J Biomol NMR, 2004 Mar. PMID 14752268

See all (44) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines
    Title: Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA.
  2. The uridylyltransferase GlnD and tRNA modification GTPase MnmE allosterically control Escherichia coli folylpoly-gamma-glutamate synthase FolC.
    Title: The uridylyltransferase GlnD and tRNA modification GTPase MnmE allosterically control Escherichia coli folylpoly-γ-glutamate synthase FolC.
  3. Investigation of the activities and the tRNA specificity of the MnmE enzyme.
    Title: The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species.
  4. GidA binding induces large conformational and dynamic changes in MnmE
    Title: Stabilization of G domain conformations in the tRNA-modifying MnmE-GidA complex observed with double electron electron resonance spectroscopy.
  5. The MnmE*GidA complex catalyzes two different GTP- and FAD-dependent reactions, which produce 5-aminomethyluridine and 5-carboxymethylamino-methyluridine using ammonium and glycine, respectively, as substrates.
    Title: Evolutionarily conserved proteins MnmE and GidA catalyze the formation of two methyluridine derivatives at tRNA wobble positions.
  6. Hydrolysis of GTP and intact complex formation in the MnmE-GidA complex is essential for the in vitro and in in vivo tRNA-modification activity which employs glycine as a substrate.
    Title: G-domain dimerization orchestrates the tRNA wobble modification reaction in the MnmE/GidA complex.
  7. Biochemical and X-ray structural evidence provided for a new GTP-hydrolysis mechanism, where the G-domains of MnmE dimerise in a potassium-dependent manner and induce GTP hydrolysis.
    Title: Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element.
  8. Mutational analysis of all conserved arginine residues of the MnmE G-domain indicates that MnmE, unlike other GTPases, does not use an arginine finger to drive catalysis.
    Title: Effects of mutagenesis in the switch I region and conserved arginines of Escherichia coli MnmE protein, a GTPase involved in tRNA modification.
  9. Data suggest that TrmE is part of an increasingly complex control network designed to integrate diverse physiological signals and forecast future exposures to extreme acid.
    Title: The Era-like GTPase TrmE conditionally activates gadE and glutamate-dependent acid resistance in Escherichia coli.
  10. MnmE and GidA form a functional complex in the tRNA modification process in which both proteins are interdependent.
    Title: Further insights into the tRNA modification process controlled by proteins MnmE and GidA of Escherichia coli.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables GDP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables potassium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within chaperone-mediated protein folding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of cytoplasmic translational fidelity NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA wobble uridine modification IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA wobble uridine modification IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within tRNA wobble uridine modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic tRNA wobble base thiouridylase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3886828..3888192
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418162.1 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418162.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7RYS3
    Conserved Domains (1) summary
    PRK05291
    Location:1 → 454
    trmE; tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE
Nucleotide Protein
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