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    pknB serine/threonine-protein kinase PknB [ Mycobacterium tuberculosis H37Rv ]

    Gene ID: 887072, updated on 6-Oct-2023

    Summary

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    Gene symbol
    pknB
    Gene description
    serine/threonine-protein kinase PknB
    Locus tag
    Rv0014c
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mycobacterium tuberculosis H37Rv (strain: H37Rv, type-material: type strain of Mycobacterium tuberculosis)
    Lineage
    Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex
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    Genomic context

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    Sequence:
    NC_000962.3 (15590..17470, complement)

    NC_000962.3Genomic Context describing neighboring genes Neighboring gene cell division protein CrgA Neighboring gene membrane protein Neighboring gene anthranilate synthase component II Neighboring gene serine/threonine-protein kinase PknA Neighboring gene penicillin-binding protein PbpA Neighboring gene cell division protein RodA Neighboring gene phosphoserine/threonine phosphatase PstP

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000962.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Protein kinase B (PknB) of Mycobacterium tuberculosis is essential for growth of the pathogen in vitro as well as for survival within the host. Chawla Y, et al. J Biol Chem, 2014 May 16. PMID 24706757, Free PMC Article
    2. Auto-activation mechanism of the Mycobacterium tuberculosis PknB receptor Ser/Thr kinase. Mieczkowski C, et al. EMBO J, 2008 Dec 3. PMID 19008858, Free PMC Article
    3. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Prisic S, et al. Proc Natl Acad Sci U S A, 2010 Apr 20. PMID 20368441, Free PMC Article
    4. Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB. Lombana TN, et al. Structure, 2010 Dec 8. PMID 21134645, Free PMC Article
    5. The extracytoplasmic domain of the Mycobacterium tuberculosis Ser/Thr kinase PknB binds specific muropeptides and is required for PknB localization. Mir M, et al. PLoS Pathog, 2011 Jul. PMID 21829358, Free PMC Article

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The protein kinase PknB negatively regulates biosynthesis and trafficking of mycolic acids in mycobacteria.
      Title: The protein kinase PknB negatively regulates biosynthesis and trafficking of mycolic acids in mycobacteria.
    2. Depletion of PknA and/or PknB resulted in markedly increased susceptibility to beta-lactam antibiotics.
      Title: Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility.
    3. The extracytoplasmic domain of M. tuberculosis PknB interacts with mDAP-containing Lipid II, and this is abolished upon mutation of the ligand-interacting residues. Abrogation of ligand-binding or sequestration of the ligand leads to aberrant localization of PknB. Abrogation of ligand-binding is linked to activation loop hyperphosphorylation, and indiscriminate hyperphosphorylation of PknB substrates causing cell death.
      Title: LipidII interaction with specific residues of Mycobacterium tuberculosis PknB extracytoplasmic domain governs its optimal activation.
    4. the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.
      Title: Inhibition of the DevSR Two-Component System by Overexpression of Mycobacterium tuberculosis PknB in Mycobacterium smegmatis.
    5. PknB is an ideal target for drug development and there is no pre-existing or induced resistance with respect to the sequences involved in inhibitor binding.
      Title: PknB remains an essential and a conserved target for drug development in susceptible and MDR strains of M. Tuberculosis.
    6. PknB activity is modulated by extracellular domain binding to peptidoglycan substructures.
      Title: Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.
    7. The PknB PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell-wall architecture.
      Title: The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth.
    8. Here, the authors demonstrate that the alpha-subunit (PrcA) of the Mycobacterium tuberculosis proteasome is phosphorylated by the PknB kinase at three threonine residues (T84, T202, and T178) in a sequential manner.
      Title: Phosphorylation regulates mycobacterial proteasome.
    9. the role of PknB in mediating pathogen survival in the host and in vitro
      Title: Protein kinase B (PknB) of Mycobacterium tuberculosis is essential for growth of the pathogen in vitro as well as for survival within the host.
    10. simulations suggest that autophosphorylation of PknB might also involve cis initiation followed by trans amplification as reported for other eukaryotic kinases based on recent reaction kinetics studies
      Title: Mechanism of autophosphorylation of mycobacterial PknB explored by molecular dynamics simulations.
    Submit: New GeneRIF Correction See all GeneRIFs (21)

    General gene information

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    Homology

    General protein information

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    Preferred Names
    serine/threonine-protein kinase PknB
    NP_214528.1
    • Belongs to the Ser/Thr family of protein kinases. Experimental studies show evidence of auto-phosphorylation on serine/threonine residues. PknB has been shown to be a substrate for PstP and its kinase activity is affected by PstP-mediated dephosphorylation. PknB and PstP (Rv0018c) may act as a functional pair in vivo to control mycobacterial cell growth.

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000962.3 Reference assembly

      Range
      15590..17470 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_214528.1 serine/threonine-protein kinase PknB [Mycobacterium tuberculosis H37Rv]

      See identical proteins and their annotated locations for NP_214528.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2I7W281
      Conserved Domains (3) summary
      smart00740
      Location:557 → 625
      PASTA; PASTA domain
      sd00037
      Location:361 → 420
      PASTA; PASTA domain [structural motif]
      NF033483
      Location:3 → 556
      PknB_PASTA_kin; Stk1 family PASTA domain-containing Ser/Thr kinase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P9WI81.1

    Gene LinkOut

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