BIOF biotin F [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: BIOF
Official Full Name: biotin F
Primary source: TAIR:AT5G04620
Locus tag: AT5G04620
See related: Araport:AT5G04620
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: 7-KETO-8-AMINOPELARGONIC ACID (KAPA) SYNTHASE; ATBIOF; BIO4; biotin 4; biotin F; T1E3.3
Summary: The cDNA encoding 7-keto-8-aminopelargonic acid (KAPA) synthase, the first committed enzyme of the biotin synthesis pathway has been cloned and its molecular function confirmed (functional complementation of an E. coli mutant). The subcellular localization of the enzyme (cytosol) proves that the biotin biosynthesis in plants takes place in different compartments which differs from the biosynthetic route found in microorganisms.
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Genomic context

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See BIOF in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 10

Sequence:: Chromosome: 5; NC_003076.8 (1327093..1330151)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that the bio4-1 plants (mutant of BIO4, At5g04620) exhibited massive accumulation of hydrogen peroxide and constitutive up-regulation of a number of genes that are diagnostic for defense and reactive oxygen species signaling.
Title: Biotin deficiency causes spontaneous cell death and activation of defense signaling.

Submit: New GeneRIF Correction

General gene information

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Homology

Orthologs from OrthoDB

NM_001342782.1

Supported by ESTs:

NM_120544.3

Supported by ESTs:

NM_203002.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 8-amino-7-oxononanoate synthase activity	IEA Inferred from Electronic Annotation more info
enables 8-amino-7-oxononanoate synthase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within biotin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in biotin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in biotin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: biotin F

NP_196082.2

biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 10202 Blast hits to 10184 proteins in 2138 species: Archae - 77; Bacteria - 6906; Metazoa - 648; Fungi - 572; Plants - 222; Viruses - 9; Other Eukaryotes - 1768 (source: NCBI BLink).

NP_974731.1

biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

1327093..1330151

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_203002.4 → NP_974731.1 biotin F [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_974731.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q2QKD2, Q8GW43, Q9LZ63

UniProtKB/TrEMBL

A0A178U769, A0A5S9Y3K8

Conserved Domains (1) summary

PLN02955
Location:1 → 476

PLN02955; 8-amino-7-oxononanoate synthase
NM_001342782.1 → NP_001332170.1 biotin F [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8BG43
NM_120544.3 → NP_196082.2 biotin F [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_196082.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8GW43

Conserved Domains (2) summary

pfam00155
Location:11 → 322

Aminotran_1_2; Aminotransferase class I and II

cl18945
Location:1 → 343

AAT_I; Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the ...