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EIF5B eukaryotic translation initiation factor 5B [ Homo sapiens (human) ]

Gene ID: 9669, updated on 14-Nov-2024

Summary

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Official Symbol
EIF5Bprovided by HGNC
Official Full Name
eukaryotic translation initiation factor 5Bprovided by HGNC
Primary source
HGNC:HGNC:30793
See related
Ensembl:ENSG00000158417 MIM:606086; AllianceGenome:HGNC:30793
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
IF2
Summary
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in heart (RPKM 26.2), testis (RPKM 23.3) and 25 other tissues See more
Orthologs
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Genomic context

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See EIF5B in Genome Data Viewer
Location:
2q11.2
Exon count:
24
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (99337389..99401326)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (99796021..99859976)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (99953852..100017789)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107985923 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16281 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99952564-99953104 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16284 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16285 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16286 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99953733-99954234 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:99954235-99954734 Neighboring gene thioredoxin domain containing 9 Neighboring gene lysozyme g1 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr2:100019841-100021040 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11808 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11809 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr2:100056357-100057026 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:100092059-100092722 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16288 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11810 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11811 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:100106417-100107302 Neighboring gene REV1 DNA directed polymerase Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16289 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100146131-100146758 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100170377-100170925 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100170926-100171473 Neighboring gene NANOG hESC enhancer GRCh37_chr2:100171857-100172363 Neighboring gene NANOG hESC enhancer GRCh37_chr2:100188674-100189175 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:100218069-100218631 Neighboring gene ALF transcription elongation factor 3 Neighboring gene uncharacterized LOC124906051 Neighboring gene uncharacterized LOC105373504

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Poliovirus switches to an eIF2-independent mode of translation during infection. White JP, et al. J Virol, 2011 Sep. PMID 21697471, Free PMC Article
  2. eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer. Suresh S, et al. Nat Cancer, 2020 May. PMID 32984844, Free PMC Article
  3. Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Ho JJD, et al. Cell Rep, 2018 Jan 2. PMID 29298419, Free PMC Article
  4. Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probing. Unbehaun A, et al. EMBO J, 2007 Jul 11. PMID 17568775, Free PMC Article
  5. Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5. Paul EE, et al. Biophys Chem, 2022 Feb. PMID 34923394, Free PMC Article

See all (169) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
    Title: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
  2. eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer.
    Title: eIF5B drives integrated stress response-dependent translation of PD-L1 in lung cancer.
  3. Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5.
    Title: Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5.
  4. Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK).
    Title: Depletion of eukaryotic initiation factor 5B (eIF5B) reprograms the cellular transcriptome and leads to activation of endoplasmic reticulum (ER) stress and c-Jun N-terminal kinase (JNK).
  5. eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1.
    Title: eIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1.
  6. Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumour stem cells (BTSCs).
    Title: Eukaryotic initiation factor 5B (eIF5B) regulates temozolomide-mediated apoptosis in brain tumour stem cells (BTSCs).
  7. Data suggest that eIF5B represents a regulatory node, allowing cancer cells to evade apoptosis by promoting the translation of pro-survival proteins from IRES-containing mRNAs.
    Title: Eukaryotic initiation factor 5B (eIF5B) provides a critical cell survival switch to glioblastoma cells via regulation of apoptosis.
  8. results indicate that in humans, eIF5B displacing eIF2 from Met-tRNAi upon subunit joining may be coupled to eIF1A displacing eIF5 from eIF5B, allowing the eIF5:eIF2-GDP complex to leave the ribosome.
    Title: Human eIF5 and eIF1A Compete for Binding to eIF5B.
  9. aerobic eukarya retained eIF5B/IF2 to remodel anaerobic pathways during episodes of oxygen deficiency.
    Title: Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.
  10. eIF5B promoted hepatocellular carcinoma cell proliferation and migration in vitro and in vivo partly through increasing ASAP1 expression.
    Title: eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
Submit: New GeneRIF Correction See all GeneRIFs (18)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag HIV-1 Gag interacts with EIF5B as demonstrated by proximity dependent biotinylation proteomics PubMed
matrix gag HIV-1 Matrix inhibits hIF2 mediated translation in a reticulocyte lysate assay PubMed
gag HIV-1 Matrix, as well as the full length HIV-1 Gag protein bind to human translation initiation factor two (hIF2) and these proteins can be immunoprecipitated as a protein complex PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ10524, KIAA0741, DKFZp434I036

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables RNA binding HDA PubMed 
enables membrane scission GTPase motor activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables tRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables translation initiation factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables translation initiation factor activity NAS
Non-traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in regulation of translational initiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of translational initiation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in ribosome assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in translational initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
NOT located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in synapse IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
eukaryotic translation initiation factor 5B
Names
eIF-5B
translation initiation factor IF-2
translation initiation factor IF2
NP_056988.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_015904.4 → NP_056988.3  eukaryotic translation initiation factor 5B

    See identical proteins and their annotated locations for NP_056988.3

    Status: REVIEWED

    Source sequence(s)
    AB018284, AC018690, BC032639, BX485839
    Consensus CDS
    CCDS42721.1
    UniProtKB/Swiss-Prot
    O60841, O95805, Q53RV7, Q53SI8, Q9UF81, Q9UMN7
    UniProtKB/TrEMBL
    Q8N5A0
    Related
    ENSP00000289371.5, ENST00000289371.11
    Conserved Domains (6) summary
    cd01887
    Location:632 → 845
    IF2_eIF5B; Initiation Factor 2 (IF2)/ eukaryotic Initiation Factor 5B (eIF5B) family
    PRK04004
    Location:627 → 1202
    PRK04004; translation initiation factor IF-2; Validated
    pfam08070
    Location:110 → 171
    DTHCT; DTHCT (NUC029) region
    pfam11987
    Location:966 → 1067
    IF-2; Translation-initiation factor 2
    cd03703
    Location:855 → 965
    aeIF5B_II; Domain II of archaeal and eukaryotic Initiation Factor 5
    cd16266
    Location:1092 → 1186
    IF2_aeIF5B_IV; Domain IV of prokaryotic Initiation Factor 2 and archaeal and eukaryotic Initiation Factor 5

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

    Range
    99337389..99401326
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060926.1 Alternate T2T-CHM13v2.0

    Range
    99796021..99859976
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O60841.4 GenPept UniProtKB/Swiss-Prot:O60841

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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