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csgD DNA-binding transcriptional dual regulator CsgD [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949119, updated on 19-Sep-2024

Summary

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Official Symbol
csgD
Official Full Name
DNA-binding transcriptional dual regulator CsgD
Primary source
ECOCYC:G6546
Locus tag
b1040
See related
ASAP:ABE-0003530
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1026
Summary
CsgD (AgfD) has also been characterized in Salmonella. [More information is available at EcoGene: EG13410]. The protein CsgD, for "Curlin subunit gene D," is a transcriptional regulator that regulates a number of genes involved in the Curli assembly, transport, and structural components , which are important for biofilm formation . [More information is available at EcoCyc: G6546].
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Genomic context

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Sequence:
NC_000913.3 (1102546..1103196, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene curli assembly component CsgF Neighboring gene curli assembly component CsgE Neighboring gene curlin, minor subunit Neighboring gene curlin, major subunit

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12. Ogasawara H, et al. Microbiology (Reading), 2020 Sep. PMID 32649279
  2. The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli. Smith DR, et al. Biomolecules, 2017 Oct 31. PMID 29088115, Free PMC Article
  3. Genome amplification and promoter mutation expand the range of csgD-dependent biofilm responses in an STEC population. Uhlich GA, et al. Microbiology (Reading), 2017 Apr. PMID 28406080
  4. Complex regulation of csgD promoter activity by global regulatory proteins. Gerstel U, et al. Mol Microbiol, 2003 Aug. PMID 12864849
  5. Regulation of the Escherichia coli csgD promoter: interplay between five transcription factors. Ogasawara H, et al. Microbiology (Reading), 2010 Aug. PMID 20466769

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12.
    Title: Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12.
  2. A conserved A/U-rich region in the csgD mRNA 5' untranslated region, which is cleaved upon binding of the small RNAs, McaS, RprA or GcvB, to sites located more than 30 nucleotides downstream.
    Title: sRNA-dependent control of curli biosynthesis in Escherichia coli: McaS directs endonucleolytic cleavage of csgD mRNA.
  3. Regulation of the master biofilm regulator, CsgD, was diverse, and the screen revealed several proteins and small RNAs (sRNA) that regulate csgD messenger RNA (mRNA) levels. Using previously published studies, we found minimal overlap between the genes affecting curli biogenesis and genes known to impact swimming or swarming motility, underlying the distinction between motile and sessile lifestyles.
    Title: The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli.
  4. Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli.
    Title: Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli.
  5. Promoter mutations in csgD that alter sigma factor utilization from RpoS to RpoD provide a unique mechanism for increasing biofilm formation, but the transition also disrupts normal rpoS-dependent CsgD regulation.
    Title: Genome amplification and promoter mutation expand the range of csgD-dependent biofilm responses in an STEC population.
  6. cAMP and CRP influence E. coli biofilms through transcriptional regulation of csgD.
    Title: The Catabolite Repressor Protein-Cyclic AMP Complex Regulates csgD and Biofilm Formation in Uropathogenic Escherichia coli.
  7. comparison of expression of genes involved in folate metabolism and the hmp gene in strains with intact csgD gene and with deletion in csgD; expression of genes for methylene tetrahydrofolate synthesis and formyltetrahydrofolate recycling was higher in cells with CsgD than those without; expression of hmp was lower in the presence of CsgD
    Title: The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12.
  8. In conclusion, Hha regulates biofilm formation in enterohemorrhagic Escherichia coli O157:H7 by differential regulation of FlhDC and CsgD, the global regulators of motility and curli production, respectively.
    Title: Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD.
  9. RprA can directly reduce csgD mRNA levels and translation, and ce transcription of csgD.
    Title: Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.
  10. The authors propose that the cell motility for planktonic growth is repressed by CsgD, thereby promoting the switch to biofilm formation.
    Title: Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription activator activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables DNA-binding transcription repressor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription cis-regulatory region binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within positive regulation of DNA-templated transcription initiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of single-species biofilm formation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of protein-DNA complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
DNA-binding transcriptional dual regulator CsgD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1102546..1103196 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415558.1 DNA-binding transcriptional dual regulator CsgD [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415558.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T6Q3S0
    Conserved Domains (1) summary
    PRK10100
    Location:1 → 216
    PRK10100; transcriptional regulator CsgD
Nucleotide Protein
Heading Accession and Version

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