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uvrA UvrABC excision nuclease subunit A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948559, updated on 19-Sep-2024

Summary

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Official Symbol
uvrA
Official Full Name
UvrABC excision nuclease subunit A
Primary source
ECOCYC:EG11061
Locus tag
b4058
See related
ASAP:ABE-0013290
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dar; dinE; ECK4050
Summary
UvrA is a subunit of the UvrABC nucleotide excision repair (NER) generalized DNA repair process. [More information is available at EcoCyc: EG11061].
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Genomic context

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Sequence:
NC_000913.3 (4271049..4273871, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF01894 family protein YjbQ Neighboring gene PF04237 family protein YjbR Neighboring gene ssDNA-binding protein Neighboring gene uncharacterized protein YjcB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Understanding the coupling between DNA damage detection and UvrA's ATPase using bulk and single molecule kinetics. Barnett JT, et al. FASEB J, 2019 Jan. PMID 30020831, Free PMC Article
  2. Isolation and characterization of functional domains of UvrA. Myles GM, et al. Biochemistry, 1991 Apr 23. PMID 1826851
  3. Evidence from extended X-ray absorption fine structure and site-specific mutagenesis for zinc fingers in UvrA protein of Escherichia coli. Navaratnam S, et al. J Biol Chem, 1989 Sep 25. PMID 2550431
  4. Superhelical stress restrained in plasmid DNA during repair synthesis initiated by the UvrA, B and C proteins in vitro. Backendorf C, et al. Nucleic Acids Res, 1989 Dec 25. PMID 2557590, Free PMC Article
  5. Sequences of Escherichia coli uvrA gene and protein reveal two potential ATP binding sites. Husain I, et al. J Biol Chem, 1986 Apr 15. PMID 3007478

See all (105) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
    Title: Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair.
  2. UV-irradiated Escherichia coli cells with a recN mutation did not exhibit increased nucleoid condensation, but chromosome replication and cytokinesis occurred. In the uvrA and recJ strains, the condensation step was delayed compared to the wild-type strain, and chromosome replication and cytokinesis did not occur.
    Title: Influence of uvrA, recJ and recN gene mutations on nucleoid reorganization in UV-treated Escherichia coli cells.
  3. suggest that UvrA uses negative cooperativity between its ATPase sites that is gated by damage recognition
    Title: Understanding the coupling between DNA damage detection and UvrA's ATPase using bulk and single molecule kinetics.
  4. Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases bacterial survival.
    Title: Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival.
  5. The movement of beta-hairpin in the third zinc-binding module of UvrA (Zn3hp) is required for damage-specific binding, UvrB loading and ATPase activities of UvrA. The conformational change of Zn3hp is controlled by ATP hydrolysis at the distal nucleotide binding site. A bi-phasic damage inspection model of UvrA was proposed in which movement of the Zn3hp plays a key role in damage recognition.
    Title: Movement of the β-hairpin in the third zinc-binding module of UvrA is required for DNA damage recognition.
  6. study visualized how UvrA and UvrB interacted with DNA individually or together by single- molecule imaging of quantum-dot-labeled proteins
    Title: Collaborative dynamic DNA scanning by nucleotide excision repair proteins investigated by single- molecule imaging of quantum-dot-labeled proteins.
  7. UvrA is induced after UV irradiation. UvrA levels decrease principally as a result of ClpXP-dependent protein degradation.
    Title: Controlled degradation by ClpXP protease tunes the levels of the excision repair protein UvrA to the extent of DNA damage.
  8. N-terminus verified by Edman degradation on complete protein
    Title: Sequences of Escherichia coli uvrA gene and protein reveal two potential ATP binding sites.
  9. In solution, two UvrB subunits bind to UvrA, most likely as part of a UvrA2B2 complex. The UvrA-induced dimerization of the C-terminal domain 4 regions of UvrB serves to shield these domains from premature UvrC binding.
    Title: Dynamics of the UvrABC nucleotide excision repair proteins analyzed by fluorescence resonance energy transfer.
  10. we examined the interaction of UvrABC with a DNA substrate containing a single G[8,5-Me]T cross-link and compared it with T[6,4]T (the 6-4 pyrimidine-pyrimidone photoproduct) and the C8 guanine adduct of N-acetyl-2-aminofluorene (AAF)
    Title: Recognition and incision of gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T by UvrABC nuclease.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables excinuclease ABC activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in SOS response IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide-excision repair IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide-excision repair NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in nucleotide-excision repair, DNA damage recognition IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair, preincision complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA repair complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of excinuclease repair complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of excinuclease repair complex IEA
Inferred from Electronic Annotation
more info
  
part_of excinuclease repair complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
UvrABC excision nuclease subunit A

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4271049..4273871 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418482.1 UvrABC excision nuclease subunit A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418482.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376ME40, A0A8S7EBZ6
    Conserved Domains (1) summary
    PRK00349
    Location:1 → 940
    uvrA; excinuclease ABC subunit UvrA
Nucleotide Protein
Heading Accession and Version

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