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ilvN acetohydroxy acid synthase I subunit IlvN [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948183, updated on 10-Oct-2024

Summary

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Official Symbol
ilvN
Official Full Name
acetohydroxy acid synthase I subunit IlvN
Primary source
ECOCYC:EG10502
Locus tag
b3670
See related
ASAP:ABE-0011997
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3661
Summary
Bifunctional acetohydroxybutanoate synthase / acetolactate synthase (IlvB/N) carries out both the first step in valine biosynthesis and the second step in isoleucine biosynthesis. [More information is available at EcoCyc: EG10502].
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Genomic context

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Sequence:
NC_000913.3 (3850802..3851092, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene inner membrane protein sensing glucose-6-phosphate Neighboring gene sensory histidine kinase UhpB Neighboring gene DNA-binding transcriptional activator UhpA Neighboring gene acetohydroxy acid synthase I subunit IlvB Neighboring gene ilvBN operon leader peptide

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases. Bansal A, et al. Biochemistry, 2019 Apr 16. PMID 30887800, Free PMC Article
  2. Cloning and expression of the ilvB gene of Escherichia coli K-12. Newman T, et al. Mol Gen Genet, 1982. PMID 6181375
  3. Mutant of Escherichia coli K-12 missing acetolactate synthase activity. Guardiola J, et al. J Bacteriol, 1974 Oct. PMID 4608700, Free PMC Article
  4. Purification and subunit composition of acetohydroxyacid synthase I from Escherichia coli K-12. Eoyang L, et al. J Bacteriol, 1984 Jan. PMID 6360995, Free PMC Article
  5. A new map location for the ilvB locus of Escherichia coli. Newman TC, et al. Genetics, 1980 Sep. PMID 7009323, Free PMC Article

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The structural and biophysical data indicate the molecular basis for differential binding of the ligands to acetohydroxy acid synthase I subunit IlvN (IlvN).
    Title: Crystallographic Structures of IlvN·Val/Ile Complexes: Conformational Selectivity for Feedback Inhibition of Aceto Hydroxy Acid Synthases.
  2. the dephospho-form of enzyme IIA(Ntr) is required for derepression of the ilvBN operon encoding acetohydroxy acid synthase I catalysing the first step common to the biosynthesis of the branched-chain amino acids.
    Title: Requirement of the dephospho-form of enzyme IIANtr for derepression of Escherichia coli K-12 ilvBN expression.
  3. AHAS I demonstrates cooperative feedback inhibition by valine, kinetics fit closely to an exclusive binding model. Formation of acetolactate by AHAS I is readily reversible & acetolactate can act as substrate for alternative AHAS I-catalyzed reactions.
    Title: Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acetolactate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables acetolactate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acetolactate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables acetolactate synthase regulator activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-valine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within L-valine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-valine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in branched-chain amino acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in branched-chain amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in isoleucine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within isoleucine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in isoleucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of acetolactate synthase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of acetolactate synthase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
acetohydroxy acid synthase I subunit IlvN
NP_418126.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3850802..3851092 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418126.1 acetohydroxy acid synthase I subunit IlvN [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418126.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AAN4SWK7
    Conserved Domains (1) summary
    PRK08178
    Location:1 → 96
    PRK08178; acetolactate synthase 1 small subunit
Nucleotide Protein
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