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gadE DNA-binding transcriptional activator GadE [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948023, updated on 19-Sep-2024

Summary

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Official Symbol
gadE
Official Full Name
DNA-binding transcriptional activator GadE
Primary source
ECOCYC:EG11544
Locus tag
b3512
See related
ASAP:ABE-0011475
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3496; yhiE; yhiT
Summary
The 750 bp region upstream of gadE is termed the Sensory Integration Region and contains sites needed for the regulation of three promoters (P1, -124; P2, -324/317; P3, -566) by activators YdeO, GadX, an a proposed unknown MnmE-dependent regulator, as well as controlling activation by GadE, GadW and EvgA; no Crp regulation or shorter transcripts were observed (Sayed, 2009). [More information is available at EcoGene: EG11544]. GadE is involved in the resistance to low pH observed upon overexpression of evgA or ydeO . [More information is available at EcoCyc: EG11544].
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Genomic context

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Sequence:
NC_000913.3 (3658366..3658893)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene acid-resistance membrane protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene multidrug efflux pump membrane fusion protein MdtE

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. [Structure function relationship of the LysR type transcriptional regulator]. Senda T, et al. Tanpakushitsu Kakusan Koso, 2005 Oct. PMID 16218453
  2. Overexpression of an antisense RNA, ArrS, increases the acid resistance of Escherichia coli. Aiso T, et al. Microbiology (Reading), 2014 May. PMID 24600026
  3. A 750 bp sensory integration region directs global control of the Escherichia coli GadE acid resistance regulator. Sayed AK, et al. Mol Microbiol, 2009 Mar. PMID 19220752
  4. A role for Lon protease in the control of the acid resistance genes of Escherichia coli. Heuveling J, et al. Mol Microbiol, 2008 Jul. PMID 18630346
  5. The Era-like GTPase TrmE conditionally activates gadE and glutamate-dependent acid resistance in Escherichia coli. Gong S, et al. Mol Microbiol, 2004 Nov. PMID 15522079

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These results show how GadEWX simultaneously coordinate many cellular processes to produce the overall response of E. coli to acid stress.
    Title: Decoding genome-wide GadEWX-transcriptional regulatory networks reveals multifaceted cellular responses to acid stress in Escherichia coli.
  2. These results suggest that overexpression of ArrS positively regulates gadE expression in a post-transcriptional manner.
    Title: Overexpression of an antisense RNA, ArrS, increases the acid resistance of Escherichia coli.
  3. RcsB is required for inducible acid resistance and acts at gadE-dependent and -independent promoters.
    Title: RcsB is required for inducible acid resistance in Escherichia coli and acts at gadE-dependent and -independent promoters.
  4. activation of gadA transcription requires a GAD box and binding of an RcsB/GadE heterodimer.
    Title: Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE.
  5. Three gadE promoters are located within the sensory integration region.
    Title: A 750 bp sensory integration region directs global control of the Escherichia coli GadE acid resistance regulator.
  6. The effects of sodium on the acid resistance system of GadA/BC expression appears to be through the modulation of GadX and GadW activity, which in turn controls GadE expression.
    Title: Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE.
  7. The AraC-family regulators GadX and GadW, previously found to activate gadA/BC in vitro, are now shown in vivo to directly activate gadE expression, which, in turn, activates the gadA/BC genes.
    Title: The Escherichia coli AraC-family regulators GadX and GadW activate gadE, the central activator of glutamate-dependent acid resistance.
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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cellular stress response to acidic pH IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
part_of transcription regulator complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
DNA-binding transcriptional activator GadE

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3658366..3658893
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417969.1 DNA-binding transcriptional activator GadE [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417969.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ATQ2
    Conserved Domains (1) summary
    PRK05826
    Location:69 → 104
    PRK05826; pyruvate kinase; Provisional
Nucleotide Protein
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