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guaB inosine 5'-monophosphate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946985, updated on 10-Oct-2024

Summary

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Official Symbol
guaB
Official Full Name
inosine 5'-monophosphate dehydrogenase
Primary source
ECOCYC:EG10421
Locus tag
b2508
See related
ASAP:ABE-0008257
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK2504; guaR
Summary
De novo biosyntheis of guanine nucleotides is rate-limited by IMP. [More information is available at EcoGene: EG10421]. IMP dehydrogenase (IMPDH) is a homotetramer . [More information is available at EcoCyc: EG10421].
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Genomic context

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Sequence:
NC_000913.3 (2632604..2634070, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene nalidixic acid resistance protein YfgI Neighboring gene GMP synthetase Neighboring gene exodeoxyribonuclease VII subunit XseA Neighboring gene zinc ribbon domain-containing protein YfgJ Neighboring gene 50S ribosomal subunit stability factor

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Asp338 controls hydride transfer in Escherichia coli IMP dehydrogenase. Kerr KM, et al. Biochemistry, 2000 Aug 15. PMID 10933797
  2. Complementation in vitro between guaB mutants of Escherichia coli K12. Gilbert HJ, et al. J Gen Microbiol, 1980 Mar. PMID 6104688
  3. Active-site modification of native and mutant forms of inosine 5'-monophosphate dehydrogenase from Escherichia coli K12. Gilbert HJ, et al. Biochem J, 1980 Nov 1. PMID 6112982, Free PMC Article
  4. Dual control of the gua operon of Escherichia coli K12 by adenine and guanine nucleotides. Mehra RK, et al. J Gen Microbiol, 1981 Mar. PMID 6119351
  5. Construction and characterization of guaB-lacZ fusions in Escherichia coli K12. Spibey N, et al. J Gen Microbiol, 1981 Oct. PMID 6461728

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that the CRP-cyclic AMP (cAMP) complex binds to a site located over 100 bp upstream of the guaB transcription start site, where it serves to downregulate P(guaB).
    Title: Downregulation of the Escherichia coli guaB promoter by upstream-bound cyclic AMP receptor protein.
  2. Binding of FIS to binding sites centred near positions -11, +8 and +29 relative to the guaB transcription start site results in repression of guaB promoter in vitro but not in vivo, and deletion of the fis gene increased guaB promoter activity in vivo
    Title: Downregulation of the Escherichia coli guaB promoter by FIS.
  3. The CBS subdomain of IMPDH may coordinate the activities of the enzymes of purine nucleotide metabolism and is essential for maintaining the normal ATP and GTP pool sizes in E. coli.
    Title: The CBS subdomain of inosine 5'-monophosphate dehydrogenase regulates purine nucleotide turnover.
  4. N-terminus verified by Edman degradation on complete protein
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables C-rich single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables IMP dehydrogenase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables IMP dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables IMP dehydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in GMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within GMP biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in GTP biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in purine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to UV IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
inosine 5'-monophosphate dehydrogenase
NP_417003.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2632604..2634070 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417003.1 inosine 5'-monophosphate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417003.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AAN3TRY1
    Conserved Domains (1) summary
    PRK05567
    Location:1 → 487
    PRK05567; inosine 5'-monophosphate dehydrogenase; Reviewed
Nucleotide Protein
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