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mukB chromosome partitioning protein MukB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945549, updated on 19-Sep-2024

Summary

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Official Symbol
mukB
Official Full Name
chromosome partitioning protein MukB
Primary source
ECOCYC:EG10618
Locus tag
b0924
See related
ASAP:ABE-0003138
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0915
Summary
Condensin is required for chromosome partitioning and compaction. [More information is available at EcoGene: EG10618]. The MukB protein is involved in chromosome condensation and partitioning. [More information is available at EcoCyc: EG10618].
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Genomic context

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Sequence:
NC_000913.3 (976326..980786)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative phosphotransferase YcbJ Neighboring gene envelope biogenesis factor Neighboring gene tRNA cmo(5)U34 methyltransferase Neighboring gene chromosome partitioning protein MukF Neighboring gene chromosome partitioning protein MukE Neighboring gene L,D-transpeptidase LdtD Neighboring gene peptidoglycan L,D-endopeptidase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops. Kumar R, et al. J Biol Chem, 2017 Oct 13. PMID 28842486, Free PMC Article
  2. The MukB-topoisomerase IV interaction is required for proper chromosome compaction. Kumar R, et al. J Biol Chem, 2017 Oct 13. PMID 28842485, Free PMC Article
  3. In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA. Niki H, et al. Sci Rep, 2016 Jul 8. PMID 27387439, Free PMC Article
  4. The crystal structure of the hinge domain of the Escherichia coli structural maintenance of chromosomes protein MukB. Li Y, et al. J Mol Biol, 2010 Jan 8. PMID 19853611, Free PMC Article
  5. Identification of interacting regions within the coiled coil of the Escherichia coli structural maintenance of chromosomes protein MukB. Li Y, et al. J Mol Biol, 2009 Aug 7. PMID 19482037

See all (91) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
    Title: Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
  2. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
    Title: Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
  3. Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
    Title: Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
  4. Complex formation requires functional interactions between the C- and N-terminal domains of MukF with the MukB head and neck, respectively, and MukE, which organizes the complexes by stabilizing binding of MukB heads to MukF. In the absence of head engagement, a MukF dimer bound by MukE forms complexes containing only a dimer of MukB.
    Title: Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.
  5. The authors show that a 4-helix bundle adjacent to the MukF dimerisation domain interacts functionally with the MukB coiled-coiled 'neck' adjacent to the ATPase head. They propose that this interaction leads to an asymmetric tripartite complex, as in other SMC (Structural Maintenance of Chromosomes) complexes.
    Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.
  6. Study reports that MukB binds topologically to DNA in vitro, and this binding is preferentially single-stranded DNA (ssDNA) rather than double-stranded DNA. The binding of MukB to ssDNA does not require ATP but the thermal energy enhances the binding.
    Title: In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA.
  7. MukB hinge region binds DNA and that this region of the protein is involved in sequestration of supercoils. Cells carrying mutations in the MukB hinge that reduce DNA condensation in vitro exhibit nucleoid decondensation in vivo.
    Title: The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops.
  8. Cells carrying a mukB mutant allele that encodes a protein that does not interact with topoisomerase IV exhibit severe nucleoid decompaction leading to chromosome segregation defects. These findings suggest that the MukB-topoisomerase IV complex may provide a scaffold for DNA condensation.
    Title: The MukB-topoisomerase IV interaction is required for proper chromosome compaction.
  9. activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
    Title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
  10. MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation.
    Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.
Submit: New GeneRIF Correction See all GeneRIFs (22)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chromosome condensation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chromosome condensation IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome condensation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chromosome condensation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in chromosome segregation IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within sister chromatid cohesion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of condensin complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of condensin complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoid IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
chromosome partitioning protein MukB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    976326..980786
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415444.1 chromosome partitioning protein MukB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415444.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376FNT7, A0A793J1H8, A0A8S7TEG9
    Conserved Domains (1) summary
    COG3096
    Location:1 → 1480
    MukB; Chromosome condensin MukBEF, ATPase and DNA-binding subunit MukB [Escherichia coli str. K-12 substr. MG1655 [Cell cycle control, cell division, chromosome partitioning]
Nucleotide Protein
Heading Accession and Version

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