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dxs 1-deoxy-D-xylulose-5-phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945060, updated on 10-Oct-2024

Summary

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Official Symbol
dxs
Official Full Name
1-deoxy-D-xylulose-5-phosphate synthase
Primary source
ECOCYC:G6237
Locus tag
b0420
See related
ASAP:ABE-0001461
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0414; yajP
Summary
1-deoxyxylulose-5-phosphate synthase (Dxs) catalyzes the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, as well as feeding into the pyridoxal 5-phosphate and thiamine biosynthesis pathways. [More information is available at EcoCyc: G6237].
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Genomic context

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Sequence:
NC_000913.3 (438315..440177, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene thiamine monophosphate kinase Neighboring gene phosphatidylglycerophosphatase A Neighboring gene 1-deoxyxylulose-5-phosphate synthase YajO Neighboring gene geranyl diphosphate/farnesyl diphosphate synthase Neighboring gene exodeoxyribonuclease VII subunit XseB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Revealing substrate promiscuity of 1-deoxy-D-xylulose 5-phosphate synthase. Brammer LA, et al. Org Lett, 2009 Oct 15. PMID 19778006, Free PMC Article
  2. Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. Strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis. Kuzuyama T, et al. J Bacteriol, 2000 Feb. PMID 10648511, Free PMC Article
  3. Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase. Matthews PD, et al. Appl Microbiol Biotechnol, 2000 Apr. PMID 10803894
  4. Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Jones KL, et al. Metab Eng, 2000 Oct. PMID 11120644
  5. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Kim SW, et al. Biotechnol Bioeng, 2001 Feb 20. PMID 11180061

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. H49 and H299 of dxs are critical for the closed, predecarboxylation state providing the first direct link between catalysis and conformational dynamics.
    Title: Active Site Histidines Link Conformational Dynamics with Catalysis on Anti-Infective Target 1-Deoxy-d-xylulose 5-Phosphate Synthase.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 1-deoxy-D-xylulose-5-phosphate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 1-deoxy-D-xylulose-5-phosphate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 1-deoxy-D-xylulose-5-phosphate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables thiamine pyrophosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables thiamine pyrophosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transketolase or transaldolase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in 1-deoxy-D-xylulose 5-phosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in carbohydrate derivative metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in isoprenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within isoprenoid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in organophosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphate-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within pyridoxine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in terpenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within thiamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within ubiquinone biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
1-deoxy-D-xylulose-5-phosphate synthase
NP_414954.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    438315..440177 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414954.1 1-deoxy-D-xylulose-5-phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414954.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7D7KHM4, A0A828P4H5, A0A8S7I7P8
    Conserved Domains (1) summary
    TIGR00204
    Location:11 → 620
    dxs; 1-deoxy-D-xylulose-5-phosphate synthase
Nucleotide Protein
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