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leuB 3-isopropylmalate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944798, updated on 10-Oct-2024

Summary

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Official Symbol
leuB
Official Full Name
3-isopropylmalate dehydrogenase
Primary source
ECOCYC:EG11577
Locus tag
b0073
See related
ASAP:ABE-0000265
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0075
Summary
3-isopropylmalate dehydrogenase (LeuB) carries out the third step in leucine biosynthesis, catalyzing the conversion of 3-isopropylmalate to 2-isopropyl-3-oxosuccinate. [More information is available at EcoCyc: EG11577].
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Genomic context

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Sequence:
NC_000913.3 (80867..81958, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-isopropylmalate dehydratase subunit LeuD Neighboring gene 3-isopropylmalate dehydratase subunit LeuC Neighboring gene 2-isopropylmalate synthase Neighboring gene leu operon leader peptide

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus. Wallon G, et al. J Mol Biol, 1997 Mar 14. PMID 9086278
  2. Pervasive cryptic epistasis in molecular evolution. Lunzer M, et al. PLoS Genet, 2010 Oct 21. PMID 20975933, Free PMC Article
  3. A link between hinge-bending domain motions and the temperature dependence of catalysis in 3-isopropylmalate dehydrogenase. Hajdú I, et al. Biophys J, 2009 Jun 17. PMID 19527660, Free PMC Article
  4. Rates of unfolding, rather than refolding, determine thermal stabilities of thermophilic, mesophilic, and psychrotrophic 3-isopropylmalate dehydrogenases. Gráczer E, et al. Biochemistry, 2007 Oct 16. PMID 17887729
  5. Hypermutation in derepressed operons of Escherichia coli K12. Wright BE, et al. Proc Natl Acad Sci U S A, 1999 Apr 27. PMID 10220423, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on d-malate.
    Title: Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on d-malate.
  2. Data show that residues that affect function are scattered haphazardly throughout the IMDH structure.
    Title: Pervasive cryptic epistasis in molecular evolution.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3-isopropylmalate dehydrogenase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3-isopropylmalate dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3-isopropylmalate dehydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables NAD binding IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables manganese ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-leucine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within L-leucine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-leucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-leucine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within cellular response to amino acid starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol EXP
Inferred from Experiment
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
3-isopropylmalate dehydrogenase
NP_414615.4

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    80867..81958 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414615.4 3-isopropylmalate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414615.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1X3JNC7, A0AAN3N9R9, A0AAP8BA61
    Conserved Domains (1) summary
    PRK00772
    Location:3 → 362
    PRK00772; 3-isopropylmalate dehydrogenase; Provisional
Nucleotide Protein
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