GLC7 type 1 serine/threonine-protein phosphatase catalytic subunit GLC7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GLC7
Official Full Name: type 1 serine/threonine-protein phosphatase catalytic subunit GLC7
Primary source: SGD:S000000935
Locus tag: YER133W
See related: AllianceGenome:SGD:S000000935; FungiDB:YER133W; VEuPathDB:YER133W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CID1; DIS2
Summary: Enables protein serine/threonine phosphatase activity. Involved in several processes, including DNA metabolic process; negative regulation of cell cycle phase transition; and positive regulation of cellular component organization. Acts upstream of or within positive regulation of exit from mitosis. Located in several cellular components, including kinetochore; mating projection base; and spindle pole body. Part of mRNA cleavage and polyadenylation specificity factor complex and protein phosphatase type 1 complex. Used to study cancer. Human ortholog(s) of this gene implicated in Noonan syndrome-like disorder with loose anagen hair 2. Orthologous to several human genes including PPP1CA (protein phosphatase 1 catalytic subunit alpha) and PPP1CC (protein phosphatase 1 catalytic subunit gamma). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See GLC7 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 2

Sequence:: Chromosome: V; NC_001137.3 (432495..433958)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast.
Title: Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast.
Protein phosphatase 1 in association with Bud14 inhibits mitotic exit in Saccharomyces cerevisiae.
Title: Protein phosphatase 1 in association with Bud14 inhibits mitotic exit in Saccharomyces cerevisiae.
recruitment of PP1 to Spc105/Knl1 is carefully regulated to ensure that chromosome biorientation precedes SAC silencing, thereby ensuring accurate chromosome segregation
Title: Delineating the contribution of Spc105-bound PP1 to spindle checkpoint silencing and kinetochore microtubule attachment regulation.
While ubiquitylation likely regulates Ipl1 protein stability via the canonical proteasomal degradation pathway, a non-canonical ubiquitin-dependent pathway maintains normal Glc7 localization and activity.
Title: New ubiquitin-dependent mechanisms regulating the Aurora B-protein phosphatase 1 balance in Saccharomyces cerevisiae.
Rif1 controls telomere length by recruiting PP1 to directly suppress telomerase-mediated TG repeat lengthening.
Title: Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast.
Genetic analysis suggests that a Gip1-Glc7 phosphatase complex regulates prospore membrane extension in parallel to the previously reported Vps13, Spo71, Spo73 pathway.
Title: Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.
We deleted the novel intronic RNA structure within the GLC7 intron and showed that this region, rather than the intron itself, is responsible for the cell's ability to respond to salt stress. We also showed a direct association between the in cis presence of the intronic RNA and GLC7 expression
Title: Novel Intronic RNA Structures Contribute to Maintenance of Phenotype in Saccharomyces cerevisiae.
These data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised. Finally, we show that Rif1-Glc7, unlike Sir2, has an important effect on origin firing outside of the rDNA locus that serves to prevent activation of the DNA replication checkpoint
Title: Budding Yeast Rif1 Controls Genome Integrity by Inhibiting rDNA Replication.
Data link Rif1 to negative regulation of replication origin firing through recruitment of the Glc7 phosphatase.
Title: Rif1 controls DNA replication timing in yeast through the PP1 phosphatase Glc7.
Replacement of the N terminus of eIF2gamma with the GLC7-binding domain from GAC1 or fusion of heterologous dimerization domains to eIF2gamma and GLC7, respectively, maintained eIF2alpha phosphorylation at basal levels
Title: Protein phosphatase PP1/GLC7 interaction domain in yeast eIF2γ bypasses targeting subunit requirement for eIF2α dephosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (28)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables MAP kinase serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables calmodulin-dependent protein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS140 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA replication checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ascospore formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycogen metabolic process	IEA Inferred from Electronic Annotation more info
involved_in intracellular monoatomic ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IPI Inferred from Physical Interaction more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of clathrin-dependent endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of exit from mitosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell budding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell shape	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of glycogen metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization to cell division site involved in cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to unfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell division site	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
part_of mRNA cleavage and polyadenylation specificity factor complex	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection base	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein phosphatase type 1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of protein phosphatase type 1 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: type 1 serine/threonine-protein phosphatase catalytic subunit GLC7

NP_011059.3

EC 3.1.3.16

Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

432495..433958

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179023.3 → NP_011059.3 TPA: type 1 serine/threonine-protein phosphatase catalytic subunit GLC7 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011059.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM39, P32598

UniProtKB/TrEMBL

A6ZR87, B3LRP8, C7GKX9, C8Z7F0, G2WCZ2, N1P3L5

Conserved Domains (1) summary

cd07414
Location:7 → 297

MPP_PP1_PPKL; PP1, PPKL (PP1 and kelch-like) enzymes, and related proteins, metallophosphatase domain