U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

MOT2 CCR4-NOT core ubiquitin-protein ligase subunit MOT2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856799, updated on 3-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
MOT2
Official Full Name
CCR4-NOT core ubiquitin-protein ligase subunit MOT2
Primary source
SGD:S000000870
Locus tag
YER068W
See related
AllianceGenome:SGD:S000000870; FungiDB:YER068W; VEuPathDB:YER068W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
NOT4; SIG1
Summary
Enables ubiquitin protein ligase activity. Involved in several processes, including nuclear-transcribed mRNA catabolic process; positive regulation of transcription elongation by RNA polymerase II; and protein ubiquitination. Located in cytoplasm. Part of CCR4-NOT core complex. Is active in cytosolic ribosome. Orthologous to human CNOT4 (CCR4-NOT transcription complex subunit 4). [provided by Alliance of Genome Resources, Nov 2024]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See MOT2 in Genome Data Viewer
Location:
chromosome: V
Exon count:
1
Sequence:
Chromosome: V; NC_001137.3 (293050..294813)

Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene Rrt13p Neighboring gene Rgi1p Neighboring gene bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase Neighboring gene ribonucleotide-diphosphate reductase subunit RNR1

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. The inactivation of KlNOT4, a Kluyveromyces lactis gene encoding a component of the CCR4-NOT complex, reveals new regulatory functions. Mazzoni C, et al. Genetics, 2005 Jul. PMID 15879504, Free PMC Article
  2. Molecular characterization of SIG1, a Saccharomyces cerevisiae gene involved in negative regulation of G-protein-mediated signal transduction. Leberer E, et al. EMBO J, 1994 Jul 1. PMID 8039500, Free PMC Article
  3. The C-terminal region of yeast ubiquitin-protein ligase Not4 mediates its cellular localization and stress response. Palermo V, et al. FEMS Microbiol Lett, 2021 Aug 17. PMID 34338747, Free PMC Article
  4. Nascent peptide-dependent translation arrest leads to Not4p-mediated protein degradation by the proteasome. Dimitrova LN, et al. J Biol Chem, 2009 Apr 17. PMID 19204001, Free PMC Article
  5. MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Cade RM, et al. Mol Cell Biol, 1994 May. PMID 8164669, Free PMC Article

See all (137) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Not4-dependent targeting of MMF1 mRNA to mitochondria limits its expression via ribosome pausing, Egd1 ubiquitination, Caf130, no-go-decay and autophagy.
    Title: Not4-dependent targeting of MMF1 mRNA to mitochondria limits its expression via ribosome pausing, Egd1 ubiquitination, Caf130, no-go-decay and autophagy.
  2. Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.
    Title: Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.
  3. Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A.
    Title: Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A.
  4. Ccr4-Not complex subunits Ccr4, Caf1, and Not4 are novel proteolysis factors promoting the degradation of ubiquitin-dependent substrates by the 26S proteasome.
    Title: Ccr4-Not complex subunits Ccr4, Caf1, and Not4 are novel proteolysis factors promoting the degradation of ubiquitin-dependent substrates by the 26S proteasome.
  5. findings demonstrate that Ccr4-Not controls the ubiquitylation and turnover of Rpb1, the largest subunit of RNAPII, during transcription arrest
    Title: Ccr4-Not maintains genomic integrity by controlling the ubiquitylation and degradation of arrested RNAPII.
  6. Not4 is required for global translational repression under nutritional limitations and especially for repression of mRNAs that cause transient ribosome stalling.
    Title: Not4-dependent translational repression is important for cellular protein homeostasis in yeast.
  7. results identify a stress-induced proteolytic pathway regulating cyclin C that requires nuclear to cytoplasmic relocalization and Not4p-mediated ubiquitylation
    Title: Oxidative-stress-induced nuclear to cytoplasmic relocalization is required for Not4-dependent cyclin C destruction.
  8. Screening a variety of mutants involved in ubiquitin-mediated proteolysis demonstrated an important role for the nuclear ubiquitin ligase Not4 in Yap1 degradation. Not4 was found to bind to Yap1 in an oxidant-stimulated fashion
    Title: Proteolytic degradation of the Yap1 transcription factor is regulated by subcellular localization and the E3 ubiquitin ligase Not4.
  9. Not4 is required for appropriate association of the Not5 subunit of the Ccr4-Not complex with polysomes and for ubiquitination of Rps7A.
    Title: Presence of Not5 and ubiquitinated Rps7A in polysome fractions depends upon the Not4 E3 ligase.
  10. Not4p phosphorylation is involved in tolerance to cellular stresses and acts in pathways parallel to BUR2 to affect stress responses in Saccharomyces cerevisiae
    Title: Phosphorylation of Not4p functions parallel to BUR2 to regulate resistance to cellular stresses in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in deadenylation-independent decapping of nuclear-transcribed mRNA IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear-transcribed mRNA catabolic process, no-go decay IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proteasomal protein catabolic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein monoubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein polyubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein polyubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of CCR4-NOT complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of CCR4-NOT complex IEA
Inferred from Electronic Annotation
more info
  
part_of CCR4-NOT core complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosolic ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
CCR4-NOT core ubiquitin-protein ligase subunit MOT2
NP_010991.3
  • Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001137.3 Reference assembly

    Range
    293050..294813
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178959.3 → NP_010991.3  TPA: CCR4-NOT core ubiquitin-protein ligase subunit MOT2 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010991.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DLX3, P34909
    UniProtKB/TrEMBL
    B3LRI5, B5VHJ8, C7GRQ0
    Conserved Domains (1) summary
    COG5175
    Location:9 → 560
    MOT2; Transcriptional repressor [Transcription]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P34909.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Molecular Biology Databases
Research Materials
Tools