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PRP22 DEAH-box ATP-dependent RNA helicase PRP22 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856732, updated on 14-Nov-2024

Summary

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Official Symbol
PRP22
Official Full Name
DEAH-box ATP-dependent RNA helicase PRP22
Primary source
SGD:S000000815
Locus tag
YER013W
See related
AllianceGenome:SGD:S000000815; FungiDB:YER013W; VEuPathDB:YER013W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables RNA helicase activity. Contributes to second spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for second transesterification step and spliceosomal complex disassembly. Part of U2-type catalytic step 2 spliceosome and U2-type post-spliceosomal complex. Orthologous to human DHX8 (DEAH-box helicase 8). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PRP22 in Genome Data Viewer
Location:
chromosome: V
Exon count:
1
Sequence:
Chromosome: V; NC_001137.3 (178841..182278)

Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene tRNA-Glu Neighboring gene proteasome core particle subunit beta 4 Neighboring gene oxygen-dependent protoporphyrinogen oxidase Neighboring gene Bud25p Neighboring gene medium-chain fatty acid-CoA ligase FAA2

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. Schwer B, et al. EMBO J, 1998 Apr 1. PMID 9524130, Free PMC Article
  2. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. Wagner JD, et al. EMBO J, 1998 May 15. PMID 9582286, Free PMC Article
  3. Functional domains of the yeast splicing factor Prp22p. Schneider S, et al. J Biol Chem, 2001 Jun 15. PMID 11283007
  4. Detecting circRNA in purified spliceosomal P complex. Shi S, et al. Methods, 2021 Dec. PMID 33577981, Free PMC Article
  5. Both catalytic steps of nuclear pre-mRNA splicing are reversible. Tseng CK, et al. Science, 2008 Jun 27. PMID 18583613

See all (82) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites.
    Title: Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites.
  2. C-terminal domain of the spliceosomal helicase Prp22
    Title: Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22.
  3. NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
    Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.
  4. Prp22 unwinds duplexes with 3' overhangs; the 3' -> 5' directionality has implications for positioning of Prp22 on the RNA target
    Title: Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent H2AZ histone chaperone activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent H3-H4 histone complex chaperone activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent activity, acting on RNA IEA
Inferred from Electronic Annotation
more info
  
enables DNA clamp loader activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables chromatin extrusion motor activity IEA
Inferred from Electronic Annotation
more info
  
enables cohesin loader activity IEA
Inferred from Electronic Annotation
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to second spliceosomal transesterification activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to second spliceosomal transesterification activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in chromatin looping IEA
Inferred from Electronic Annotation
more info
  
involved_in chromatin remodeling IEA
Inferred from Electronic Annotation
more info
  
involved_in generation of catalytic spliceosome for second transesterification step IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in generation of catalytic spliceosome for second transesterification step IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mRNA splicing, via spliceosome IEA
Inferred from Electronic Annotation
more info
  
involved_in protein-containing complex assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in ribonucleoprotein complex biogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in spliceosomal complex disassembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of U2-type catalytic step 2 spliceosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of U2-type post-spliceosomal complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of catalytic step 2 spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
DEAH-box ATP-dependent RNA helicase PRP22
NP_010929.3
  • DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001137.3 Reference assembly

    Range
    178841..182278
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178904.3 → NP_010929.3  TPA: DEAH-box ATP-dependent RNA helicase PRP22 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010929.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DLR0, P24384
    UniProtKB/TrEMBL
    B3LS25, B5VHE4, C7GKJ1, N1P5R0
    Conserved Domains (2) summary
    COG1185
    Location:1 → 423
    Pnp; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) [Translation, ribosomal structure and biogenesis]
    COG1643
    Location:456 → 1145
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P24384.1

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