U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

LAM4 Lam4p [ Saccharomyces cerevisiae S288C ]

Gene ID: 856480, updated on 2-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
LAM4
Official Full Name
Lam4p
Primary source
SGD:S000001122
Locus tag
YHR080C
See related
AllianceGenome:SGD:S000001122; FungiDB:YHR080C; VEuPathDB:YHR080C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
LTC3
Summary
Enables sterol binding activity and sterol transfer activity. Acts upstream of or within sterol transport. Located in cortical endoplasmic reticulum. Orthologous to human GRAMD1A (GRAM domain containing 1A) and GRAMD1C (GRAM domain containing 1C). [provided by Alliance of Genome Resources, Nov 2024]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See LAM4 in Genome Data Viewer
Location:
chromosome: VIII
Exon count:
1
Sequence:
Chromosome: VIII; NC_001140.6 (262801..266838, complement)

Chromosome VIII - NC_001140.6Genomic Context describing neighboring genes Neighboring gene bifunctional endoribonuclease/protein kinase IRE1 Neighboring gene Sae3p Neighboring gene Lrp1p Neighboring gene putative serine/threonine protein kinase KSP1

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Exchange of water for sterol underlies sterol egress from a StARkin domain. Khelashvili G, et al. Elife, 2019 Dec 4. PMID 31799930, Free PMC Article
  2. Structural basis of sterol binding and transport by a yeast StARkin domain. Jentsch JA, et al. J Biol Chem, 2018 Apr 13. PMID 29463678, Free PMC Article
  3. Structural insights into a StART-like domain in Lam4 and its interaction with sterol ligands. Gatta AT, et al. Biochem Biophys Res Commun, 2018 Jan 15. PMID 29274338
  4. Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites. Tong J, et al. Proc Natl Acad Sci U S A, 2018 Jan 30. PMID 29339490, Free PMC Article
  5. LAM Genes Contribute to Environmental Stress Tolerance but Sensibilize Yeast Cells to Azoles. Sokolov SS, et al. Front Microbiol, 2020. PMID 32047490, Free PMC Article

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Large-scale atomistic molecular dynamics simulations reveal that sterol egress involves widening of the fracture, penetration of water into the cavity, and consequent destabilization of the bound sterol. The simulations identify polar residues along the fracture that are important for sterol release.
    Title: Exchange of water for sterol underlies sterol egress from a StARkin domain.
  2. results suggest that Lam4S2 engages anionic membranes via a basic surface patch, enabling "head-first" entry of sterol into the binding pocket followed by partial closure of the entryway. Reversal of these steps enables sterol egress.
    Title: Structural basis of sterol binding and transport by a yeast StARkin domain.
  3. Ltc1 and Ltc3/4 function at the vacuole and plasma membrane, respectively, to create membrane domains that partition upstream regulators of the TORC1 and TORC2 signaling pathways to coordinate cellular stress responses with sterol homeostasis.
    Title: Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables sterol binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sterol binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sterol transfer activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sterol transfer activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in intracellular sterol transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within sterol transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cortical endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cortical endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum-plasma membrane contact site IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in organelle IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

Go to the top of the page Help
Preferred Names
Lam4p
NP_011948.1
  • Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001140.6 Reference assembly

    Range
    262801..266838 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179210.1 → NP_011948.1  TPA: Lam4p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011948.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DL31, P38800
    UniProtKB/TrEMBL
    A6ZSY9, B3LSJ7, N1P2P0
    Conserved Domains (2) summary
    cd13220
    Location:561 → 654
    PH-GRAM_GRAMDC; GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain
    pfam16016
    Location:761 → 910
    DUF4782; Domain of unknown function (DUF4782)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38800.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools