OPI1 transcriptional regulator OPI1 [Saccharomyces cerevisiae S288C] -

Summary

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Official Symbol: OPI1
Official Full Name: transcriptional regulator OPI1
Primary source: SGD:S000001012
Locus tag: YHL020C
See related: AllianceGenome:SGD:S000001012; FungiDB:YHL020C; VEuPathDB:YHL020C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidic acid binding activity and transcription corepressor activity. Involved in several processes, including cellular response to phosphate starvation; endoplasmic reticulum unfolded protein response; and regulation of transcription by RNA polymerase II. Located in endoplasmic reticulum; nuclear membrane; and nucleoplasm. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See OPI1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (66242..67456, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Opi1-mediated transcriptional modulation orchestrates genotoxic stress response in budding yeast.
Title: Opi1-mediated transcriptional modulation orchestrates genotoxic stress response in budding yeast.
This paper revealed evidence for a phosphatidic acid selectivity of Opi1.
Title: The molecular recognition of phosphatidic acid by an amphipathic helix in Opi1.
The work shows that transcriptional repressor Opi1p is redox-regulated and establishes a new mechanism of gene regulation involving Opi1p, which is important for adaptation to H2 O2 in yeast cells (Saccharomyces cerevisiae).
Title: Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae.
Data suggest that a specific pool of phosphatidic acid, associated with lipid droplet formation in perinuclear ER, is responsible for initial rapid exit of Opi1p from nucleus to ER and is required for Ino1 expression in presence of choline; interaction between Opi1p and Scs2p in ER membrane facilitates transit of phosphatidic acid from ER to mitochondria.
Title: Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential for INO1 gene expression in the presence of choline.
This study demonstrated that Pho85 binds to Opi1 in vitro and in vivo and that this interaction is increased in the presence of high concentrations of phosphate.
Title: Opi1 mediates repression of phospholipid biosynthesis by phosphate limitation in the yeast Saccharomyces cerevisiae.
Opi1p dissociates from Scs2p after targeting to the nuclear membrane, making it possible to be released from the membrane quickly when phosphatidic acid levels decrease.
Title: Induction of intranuclear membranes by overproduction of Opi1p and Scs2p, regulators for yeast phospholipid biosynthesis, suggests a mechanism for Opi1p nuclear translocation.
genes of phospholipid biosynthesis are negatively regulated by repressor protein Opi1 when precursor molecules inositol and choline are available. Opi1-triggered gene repression is mediated by recruitment of the Sin3 corepressor complex.
Title: Multiple histone deacetylases are recruited by corepressor Sin3 and contribute to gene repression mediated by Opi1 regulator of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
demonstrate that a physical association between the Yet complex and Scs2p-Opi1p is required for proper localization of the Opi1p repressor to endoplasmic reticulum membranes and subsequent INO1 derepression
Title: Yet1p-Yet3p interacts with Scs2p-Opi1p to regulate ER localization of the Opi1p repressor.
Mutational analysis of the Opi1-Sin3 interaction domain (OSID) revealed that hydrophobic OSID residues L56, V59 and V67 of Opi1 are indispensable for gene repression
Title: Pleiotropic corepressors Sin3 and Ssn6 interact with repressor Opi1 and negatively regulate transcription of genes required for phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
a rapid decrease in intracellular pH in response to glucose starvation regulated binding of phosphatidic acid to Opi1, that coordinately repressed phospholipid metabolic genes; this enabled coupling of membrane biogenesis to nutrient availability
Title: Phosphatidic acid is a pH biosensor that links membrane biogenesis to metabolism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables phosphatidic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription corepressor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription corepressor activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to phosphate starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in endoplasmic reticulum unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phospholipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear periphery	HDA more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcriptional regulator OPI1

NP_011843.1

Transcriptional regulator of a variety of genes; critical sensor of genotoxic stress; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

66242..67456 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179100.1 → NP_011843.1 TPA: transcriptional regulator OPI1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011843.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKP9, P21957

UniProtKB/TrEMBL

A6ZSN1, B3LS98, G2WF46, N1P306

Conserved Domains (1) summary

pfam08618
Location:131 → 361

Opi1; Transcription factor Opi1