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KAR3 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856263, updated on 22-Aug-2024

Summary

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Official Symbol
KAR3
Primary source
FungiDB:YPR141C
Locus tag
YPR141C
See related
SGD:S000006345; AllianceGenome:SGD:S000006345; VEuPathDB:YPR141C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
OSR11
Summary
Enables microtubule binding activity and minus-end-directed microtubule motor activity. Involved in several processes, including conjugation with cellular fusion; mitotic sister chromatid cohesion; and regulation of mitotic spindle organization. Located in cytoplasmic microtubule and spindle pole body. Orthologous to human KIFC1 (kinesin family member C1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See KAR3 in Genome Data Viewer
Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (815734..817923, complement)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene lysophosphatidic acid acyltransferase LOA1 Neighboring gene lysophosphatidylcholine acyltransferase Neighboring gene Rrp15p Neighboring gene tRNA-Ile

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structure of the Kar3-like kinesin motor domain from the filamentous fungus Ashbya gossypii. Duan D, et al. Proteins, 2012 Apr. PMID 22493778
  2. Structure determination of the motor domain of yeast kinesin kar3 by x-ray crystallography. Park HW. Methods Mol Biol, 2007. PMID 17951720
  3. Mechanistic analysis of the Saccharomyces cerevisiae kinesin Kar3. Mackey AT, et al. J Biol Chem, 2004 Dec 3. PMID 15385545, Free PMC Article
  4. The minus end-directed motor Kar3 is required for coupling dynamic microtubule plus ends to the cortical shmoo tip in budding yeast. Maddox PS, et al. Curr Biol, 2003 Aug 19. PMID 12932327
  5. The ATPase cross-bridge cycle of the Kar3 motor domain. Implications for single head motility. Mackey AT, et al. J Biol Chem, 2003 Feb 7. PMID 12446697

See all (146) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
    Title: The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
  2. The kinesin-14 motor protein complex (Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric double-strand breaks and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication.
    Title: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
  3. findings provide an example for a non-conventional translocation mechanism and can explain how Kar3 substitutes for key functions of Dynein in the yeast nucleus
    Title: Non-catalytic motor domains enable processive movement and functional diversification of the kinesin-14 Kar3.
  4. Cryoelectron microscopy generated 3-D structure of Kar3Cik1 complexed to microtubules in various nucleotide states.
    Title: Common mechanistic themes for the powerstroke of kinesin-14 motors.
  5. Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends.
    Title: Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.
  6. Neck rotation and neck mimic docking in Vik1 and Cik1 is a structural mechanism for communication with Kar3.
    Title: Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.
  7. Rapid ATP binding to Kar3 is associated with rotation of the coiled-coil stalk, and the postpowerstroke ATP hydrolysis is independent of Vik1.
    Title: The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.
  8. show through structural determination of a C-terminal heterodimeric Kar3Vik1, electron microscopy, equilibrium binding, and motility that at the start of the cycle, Kar3Vik1 binds to or occludes two alphabeta-tubulin subunits on adjacent protofilaments
    Title: Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.
  9. we found that Cik1 mediates the association of Kar3 with kinetochores and this association only occurs before chromosome bipolar attachment.
    Title: Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint.
  10. a model in which Kar3Cik1 interacts with the MT lattice through an alternating cycle of Cik1 MT collision followed by Kar3 MT binding with head-head communication between Kar3 and Cik1 modulated by the Kar3 nucleotide state and intramolecular strain
    Title: Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables cytoskeletal motor activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables microtubule binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables microtubule binding IEA
Inferred from Electronic Annotation
more info
  
enables microtubule motor activity IEA
Inferred from Electronic Annotation
more info
  
enables minus-end-directed microtubule motor activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome segregation IEA
Inferred from Electronic Annotation
more info
  
involved_in karyogamy IEA
Inferred from Electronic Annotation
more info
  
involved_in karyogamy involved in conjugation with cellular fusion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in meiotic cell cycle IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in meiotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in microtubule-based movement IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic sister chromatid cohesion IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic sister chromatid cohesion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear migration involved in conjugation with cellular fusion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of mitotic spindle organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic microtubule IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in microtubule HDA PubMed 
located_in microtubule IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in spindle IEA
Inferred from Electronic Annotation
more info
  
located_in spindle pole HDA PubMed 
located_in spindle pole body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle pole body IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Kar3p
NP_015467.1
  • Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    815734..817923 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184238.1 → NP_015467.1  TPA: Kar3p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015467.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W4D9, P17119
    UniProtKB/TrEMBL
    A6ZX18, B3LKA2, C8ZJG4, G2WPV5, N1NXT6
    Conserved Domains (2) summary
    COG1196
    Location:98 → 387
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    cd01366
    Location:384 → 725
    KISc_C_terminal; Kinesin motor domain, KIFC2/KIFC3/ncd-like carboxy-terminal kinesins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P17119.1

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