TAH18 NAPDH-dependent diflavin reductase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TAH18
Official Full Name: NAPDH-dependent diflavin reductase
Primary source: SGD:S000006252
Locus tag: YPR048W
See related: AllianceGenome:SGD:S000006252; FungiDB:YPR048W; VEuPathDB:YPR048W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables oxidoreductase activity, acting on NAD(P)H. Involved in several processes, including nitric oxide biosynthetic process; positive regulation of hydrogen peroxide-mediated programmed cell death; and positive regulation of nitric oxide biosynthetic process. Located in mitochondrion. Orthologous to human NDOR1 (NADPH dependent diflavin oxidoreductase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TAH18 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (659182..661053)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tah18-dependent NO production and cell death are suppressed by enhancement of the interaction between Tah18 and its molecular partner Dre2
Title: Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast.
the pathways for RNR and Fe-S cluster biogenesis bifurcate after the Dre2-Tah18 step.
Title: The diferric-tyrosyl radical cluster of ribonucleotide reductase and cytosolic iron-sulfur clusters have distinct and similar biogenesis requirements.
Gene knockdown analysis demonstrated that Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells.
Title: The flavoprotein Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells.
Interaction between the Tah18 and Dre2 C-terminus is essential for viability.
Title: Interaction between the reductase Tah18 and highly conserved Fe-S containing Dre2 C-terminus is essential for yeast viability.
These results are in favour of an oxidative stress-induced cell death in yeast that involves mitochondria and is controlled by the newly identified Dre2-Tah18 complex.
Title: A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables NADPH-hemoprotein reductase activity	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on NAD(P)H	IDA Inferred from Direct Assay more info	PubMed
enables oxidoreductase activity, acting on NAD(P)H	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in iron-sulfur cluster assembly	IEA Inferred from Electronic Annotation more info
involved_in iron-sulfur cluster assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: NAPDH-dependent diflavin reductase

NP_015373.1

Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance; possible target for development of antifungal drugs

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

659182..661053

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184145.1 → NP_015373.1 TPA: NAPDH-dependent diflavin reductase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015373.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W456, Q12181

UniProtKB/TrEMBL

A6ZWT9, B3LLA6, B5VTK2, C7GY37, C8ZJ73, N1NW17

Conserved Domains (1) summary

COG0369
Location:1 → 623

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]