U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

GLN1 glutamate--ammonia ligase [ Saccharomyces cerevisiae S288C ]

Gene ID: 856147, updated on 18-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
GLN1
Official Full Name
glutamate--ammonia ligase
Primary source
SGD:S000006239
Locus tag
YPR035W
See related
AllianceGenome:SGD:S000006239; FungiDB:YPR035W; VEuPathDB:YPR035W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables glutamate-ammonia ligase activity. Involved in glutamine biosynthetic process. Located in nuclear periphery. Human ortholog(s) of this gene implicated in Alzheimer's disease; Huntington's disease; and temporal lobe epilepsy. Orthologous to human GLUL (glutamate-ammonia ligase). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See GLN1 in Genome Data Viewer
Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (642208..643320)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene histidine--tRNA ligase Neighboring gene Arp7p Neighboring gene H(+)-transporting V1 sector ATPase subunit H Neighboring gene Spo24p

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. The glutamine synthetase of Trypanosoma cruzi is required for its resistance to ammonium accumulation and evasion of the parasitophorous vacuole during host-cell infection. Crispim M, et al. PLoS Negl Trop Dis, 2018 Jan. PMID 29320490, Free PMC Article
  2. Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly. He YX, et al. Proteins, 2009 Jul. PMID 19322816
  3. A glutamine synthetase mutant of Saccharomyces cerevisiae shows defect in cell wall. Shimizu J, et al. J Gen Appl Microbiol, 1997 Jun. PMID 12501331
  4. Glutamine synthesis is a regulatory signal controlling glucose catabolism in Saccharomyces cerevisiae. Flores-Samaniego B, et al. J Bacteriol, 1993 Dec. PMID 7902349, Free PMC Article
  5. Biochemical and physiological aspects of glutamine synthetase inactivation in Saccharomyces cerevisiae. Mitchell AP, et al. J Biol Chem, 1984 Oct 10. PMID 6148344

See all (81) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The results point to the stress-related proteins Ahp1, Yhb1 and Oye2, as well as Gln1 and Kes1 as putative candidates for being evaluated as biomarkers for diagnostic assays of S. cerevisiae infections.
    Title: Immunoproteomic profiling of Saccharomyces cerevisiae systemic infection in a murine model.
  2. Comparative structure analysis suggests that citrate binding could induce structural fluctuation of the segment Leu293-Ala300, which may serve the role of guarding the glutamate entrance to the active sites.
    Title: Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly.
  3. Data suggested that Gpx3 protects GS from non-enzymatic proteolysis, thereby contributing to cell homeostasis when cell is exposed to oxidative stress.
    Title: Glutathione peroxidase 3 of Saccharomyces cerevisiae suppresses non-enzymatic proteolysis of glutamine synthetase in an activity-independent manner.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables glutamine synthetase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamine synthetase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamine synthetase activity IEA
Inferred from Electronic Annotation
more info
  
enables glutamine synthetase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in glutamine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glutamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear periphery IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus HDA PubMed 

General protein information

Go to the top of the page Help
Preferred Names
glutamate--ammonia ligase
NP_015360.2
  • Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; forms filaments of back-to-back stacks of cylindrical homo-decamers at low pH, leading to enzymatic inactivation and storage during states of advanced cellular starvation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    642208..643320
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184132.2 → NP_015360.2  TPA: glutamate--ammonia ligase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015360.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W445, P32288, Q03959
    UniProtKB/TrEMBL
    A6ZWS8, B3LL96, C7GQA3, C8ZJ59, G2WPL3, N1P229
    Conserved Domains (1) summary
    PLN02284
    Location:19 → 352
    PLN02284; glutamine synthetase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32288.4

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools
Miscellaneous