PHO85 cyclin-dependent serine/threonine-protein kinase PHO85 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PHO85
Gene description: cyclin-dependent serine/threonine-protein kinase PHO85
Primary source: FungiDB:YPL031C
Locus tag: YPL031C
See related: SGD:S000005952; AllianceGenome:SGD:S000005952; VEuPathDB:YPL031C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LDB15
Summary: Enables cyclin-dependent protein serine/threonine kinase activity. Involved in several processes, including negative regulation of calcium-mediated signaling; regulation of cellular biosynthetic process; and regulation of macroautophagy. Located in nucleus. Part of Pho85-Pho80 CDK-cyclin complex. Used to study lissencephaly. Human ortholog(s) of this gene implicated in Alzheimer's disease and lissencephaly 7 with cerebellar hypoplasia. Orthologous to several human genes including CDK14 (cyclin dependent kinase 14); CDK15 (cyclin dependent kinase 15); and CDK17 (cyclin dependent kinase 17). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PHO85 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 2

Sequence:: Chromosome: XVI; NC_001148.4 (492018..493037, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The CDK Pho85 inhibits Whi7 Start repressor to promote cell cycle entry in budding yeast.
Title: The CDK Pho85 inhibits Whi7 Start repressor to promote cell cycle entry in budding yeast.
Redundant targeting of Isr1 by two CDKs in mitotic cells.
Title: Redundant targeting of Isr1 by two CDKs in mitotic cells.
Cyclin-dependent kinase Pho85p and its cyclins are involved in replicative lifespan through multiple pathways in yeast.
Title: Cyclin-dependent kinase Pho85p and its cyclins are involved in replicative lifespan through multiple pathways in yeast.
Study results provide evidence that phosphatidylinositol 4,5-bisphosphate modulates the activity of essential signaling effectors in response to cold and that Pho85 is a central regulator of lipid homeostasis in yeast Saccharomyces cerevisiae. Its activity connects different lipid biosynthesis pathways to endow yeast cells with the optimal lipid profile to face with thermal stress.
Title: Pho85 and PI(4,5)P(2) regulate different lipid metabolic pathways in response to cold.
Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells.
Title: Early protection to stress mediated by CDK-dependent PI3,5P(2) signaling from the vacuole/lysosome.
Pho85 and Pho4 regulated CLN2 expression under alkaline condition.
Title: The regulators of yeast PHO system participate in the transcriptional regulation of G1 cyclin under alkaline stress conditions.
with Srb10 clearing mostly inactive Gcn4 molecules at the promoter that are enriched for sumoylation of the activation domain, and Pho85 clearing molecules unbound to the UAS that include both fully functional and inactive Gcn4 species.
Title: Accumulation of a threonine biosynthetic intermediate attenuates general amino acid control by accelerating degradation of Gcn4 via Pho85 and Cdk8.
phosphorylations by protein kinase C and Pho85p-Pho80p protein kinase regulate Pah1p phosphatidate phosphatase abundance in Saccharomyces cerevisiae
Title: Cross-talk phosphorylations by protein kinase C and Pho85p-Pho80p protein kinase regulate Pah1p phosphatidate phosphatase abundance in Saccharomyces cerevisiae.
Comparison between Pho85-Pcl10, phosphorylated CDK2-cyclin A, and CDK5-p25 complexes reveals the convergent structural characteristics necessary for full kinase activity and the variations in the substrate recognition mechanism.
Title: New structural insights into phosphorylation-free mechanism for full cyclin-dependent kinase (CDK)-cyclin activity and substrate recognition.
Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast
Title: Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cyclin-dependent protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables cyclin-dependent protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G1/S transition of mitotic cell cycle	HGI more info	PubMed
involved_in G1/S transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall organization	HGI more info	PubMed
involved_in lipid homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of calcium-mediated signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of glycogen biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphate metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phospholipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle phase transition	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of establishment or maintenance of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of lipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of nucleocytoplasmic transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein localization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein stability	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	HGI more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Pho85-Pho80 CDK-cyclin complex	IDA Inferred from Direct Assay more info	PubMed
part_of cyclin-dependent protein kinase holoenzyme complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of serine/threonine protein kinase complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cyclin-dependent serine/threonine-protein kinase PHO85

NP_015294.1

EC 2.7.11.22

Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels, environmental conditions and progression through the cell cycle; involved in the hyperphosphorylation of Srl3p (Whi7p), regulating protein stability, mRNA levels and promotion of START; human lissencephaly-associated homolog CDK5 functionally complements null mutation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

492018..493037 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183845.1 → NP_015294.1 TPA: cyclin-dependent serine/threonine-protein kinase PHO85 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015294.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3Y2, P17157, Q03089, Q06888

UniProtKB/TrEMBL

A6ZWL6, B3LL33, C8ZIZ8, G2WPE7, N1NVZ4

Conserved Domains (1) summary

cl21453
Location:6 → 297

PKc_like; Protein Kinases, catalytic domain