SPP1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPP1
Primary source: SGD:S000006059
Locus tag: YPL138C
See related: AllianceGenome:SGD:S000006059; FungiDB:YPL138C; VEuPathDB:YPL138C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CPS40; SAF41
Summary: Enables chromatin binding activity and methylated histone binding activity. Contributes to histone methyltransferase activity (H3-K4 specific). Involved in histone H3-K4 methylation; regulation of nucleobase-containing compound metabolic process; and subtelomeric heterochromatin assembly. Located in cytosol and nucleus. Part of Set1C/COMPASS complex. Orthologous to human CXXC1 (CXXC finger protein 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (291366..292427, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases.
Title: The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases.
Structural basis for histone H3K4me3 recognition by the N-terminal domain of the PHD finger protein Spp1.
Title: Structural basis for histone H3K4me3 recognition by the N-terminal domain of the PHD finger protein Spp1.
results indicate that "chromosomal turnover rate" is a major molecular determinant of Spp1 function in the framework of meiotic chromatin structure that prepares recombination initiation sites for break formation.
Title: Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation.
A model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination.
Title: The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes.
Spp1 regulates the catalytic activity of the enzyme (Set1) and mediates its biological effect (meiotic DSB formation) independently of the single Set1 Complex.
Title: Spp1 at the crossroads of H3K4me3 regulation and meiotic recombination.
Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of double strand breaks formation and is associated with chromosome axes during meiosis.
Title: Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes.
findings show tethering of Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce double-strand breaks (DSB); by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis
Title: The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination.
Data show that Tyr1052, a putative Phe/Tyr switch of Set1, plays an essential role in the regulation of H3K4 trimethylation by COMPASS and that the mutation to phenylalanine (Y1052F) suppresses the loss of Cps40 in H3K4 trimethylation levels.
Title: Regulation of H3K4 trimethylation via Cps40 (Spp1) of COMPASS is monoubiquitination independent: implication for a Phe/Tyr switch by the catalytic domain of Set1.
the origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiae
Title: Origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to histone H3K4 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to histone H3K4 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables methylated histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables methylated histone binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Set1C/COMPASS complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Set1C/COMPASS complex	IEA Inferred from Electronic Annotation more info
part_of Set1C/COMPASS complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Spp1p

NP_015187.1

Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; promotes meiotic DSB formation by interacting with H3K4me3 and Rec107p, a protein required for Spo11p-catalyzed DSB formation located on chromosome axes; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to cytosol in response to hypoxia