TIM23 protein transporter TIM23 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TIM23
Official Full Name: protein transporter TIM23
Primary source: SGD:S000005300
Locus tag: YNR017W
See related: AllianceGenome:SGD:S000005300; FungiDB:YNR017W; VEuPathDB:YNR017W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MAS6; MIM23; MPI3
Summary: Enables mitochondrion targeting sequence binding activity and protein transmembrane transporter activity. Involved in protein import into mitochondrial matrix. Located in mitochondrion. Part of TIM23 mitochondrial import inner membrane translocase complex. Orthologous to several human genes including TIMM23 (translocase of inner mitochondrial membrane 23). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TIM23 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (662913..663581)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes.
Title: Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes.
The Mgr2 subunit of the TIM23 complex regulates membrane insertion of marginal stop-transfer signals in the mitochondrial inner membrane.
Title: The Mgr2 subunit of the TIM23 complex regulates membrane insertion of marginal stop-transfer signals in the mitochondrial inner membrane.
A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23.
Title: A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23.
Pam18 prevents lateral precursor transport from the TIM23 mitochondrial inner membrane translocase and thus traps polypeptide in a translocase-spanning manner
Title: Motor recruitment to the TIM23 channel's lateral gate restricts polypeptide release into the inner membrane.
Cation selectivity of Tim23 is crucial for efficient protein import.
Title: Cation selectivity of the presequence translocase channel Tim23 is crucial for efficient protein import.
Using site-specific in vivo crosslinking and genetic approaches in Saccharomyces cerevisiae, the authors found that both domains of Tim44 interact with the major matrix-exposed loop of Tim23, with the C-terminal domain (CTD) binding Tim17 as well.
Title: Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23.
reduced phosphatidylcholine levels differentially affect the TIM22 and TIM23 complexes in mitochondrial protein transport
Title: Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria.
These data suggest that the translocation channel and the import motor of the TIM23 complex communicate through rearrangements of the two domains of Tim44 that are stimulated by translocating proteins.
Title: Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein.
The authors report that subunit e of the F1Fo-ATP synthase, a small single-spanning inner membrane protein that is critical for inner membrane organization, is imported by TIM23 in a process that does not require activation by the membrane potential.
Title: Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential.
Tim23 contains multiple sites to interact with the intermembrane space domain of Tim21 and to bind to Tim21, Tim50, and Tom22.A central role of the intermembrane space domain of Tim23 in the formation and regulation of the presequence translocase.
Title: Molecular basis of the dynamic structure of the TIM23 complex in the mitochondrial intermembrane space.

Submit: New GeneRIF Correction See all GeneRIFs (39)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mitochondrion targeting sequence binding	IDA Inferred from Direct Assay more info	PubMed
enables mitochondrion targeting sequence binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables protein transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in protein import into mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IEA Inferred from Electronic Annotation more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of TIM23 mitochondrial import inner membrane translocase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TIM23 mitochondrial import inner membrane translocase complex	IEA Inferred from Electronic Annotation more info
part_of TIM23 mitochondrial import inner membrane translocase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein transporter TIM23

NP_014414.3

Essential core component of the TIM23 complex; subunit of the translocase of the inner mitochondrial membrane involved in protein import into the mitochondrial matrix and inner membrane; provides a structural role, contributing to the architecture of the import channel; functions as a voltage sensor and pre-sequence receptor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

662913..663581

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183194.3 → NP_014414.3 TPA: protein transporter TIM23 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014414.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1J2, P32897

UniProtKB/TrEMBL

A6ZS91, B3LPM5, C7GLN8, C8ZFP4, G2WL74, N1NZP8

Conserved Domains (1) summary

TIGR00983
Location:61 → 212

3a0801s02tim23; mitochondrial import inner membrane translocase subunit tim23