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TCB2 tricalbin [ Saccharomyces cerevisiae S288C ]

Gene ID: 855637, updated on 3-Nov-2024

Summary

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Official Symbol
TCB2
Official Full Name
tricalbin
Primary source
SGD:S000005031
Locus tag
YNL087W
See related
AllianceGenome:SGD:S000005031; FungiDB:YNL087W; VEuPathDB:YNL087W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables lipid binding activity. Involved in endoplasmic reticulum membrane organization and regulation of phosphatidylinositol dephosphorylation. Acts upstream of or within ER to Golgi ceramide transport and phospholipid homeostasis. Located in cellular bud and cortical endoplasmic reticulum. Orthologous to human ESYT1 (extended synaptotagmin 1) and ESYT2 (extended synaptotagmin 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See TCB2 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
1
Sequence:
Chromosome: XIV; NC_001146.8 (462411..465947)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Rho family GTPase RHO2 Neighboring gene DNA topoisomerase 2 Neighboring gene Snn1p Neighboring gene Mkt1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae. Brennan TC, et al. Appl Environ Microbiol, 2015 May 15. PMID 25746998, Free PMC Article
  2. ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion. Smith M, et al. J Cell Biol, 2024 Dec 2. PMID 39302311,
  3. Beta-strand recombination in tricalbin evolution and the origin of synaptotagmin-like C2 domains. Jiménez JL, et al. Proteins, 2007 Aug 15. PMID 17510957
  4. The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family. Schulz TA, et al. Biochemistry, 2004 Apr 6. PMID 15049706
  5. Tricalbin proteins regulate plasma membrane phospholipid homeostasis. Thomas FB, et al. Life Sci Alliance, 2022 Aug. PMID 35440494, Free PMC Article

See all (58) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Tricalbins function as multimers in membrane-trafficking events. TCB2 localizes first to the cell surface, and then to intracellular vesicles near the vacuole. tcb2 mutants have altered vacuole morphology and are hypersensitive to cyclohexamide.
    Title: Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking.
  2. TCB2 likely does not bind lipids in response to calcium signaling. Suggested that calcium-insensitive TCB2 may mediate the creation of hetero-oligomeric tricalbin complexes in which TCB1, TCB3, or both supply a calcium-dependent membrane binding activity.
    Title: The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family.
  3. GFP-fusion protein migrates from the cell surface to intracellular vesices near the vacuole. Binds the related proteins Tcb1 and Tcb3. Binds Pdr1p Knockout phenotype: Hypersensitivity to cycloheximide; Suppressed by RSP5.
    Title: Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables lipid binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in ER to Golgi ceramide transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within ER to Golgi ceramide transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endoplasmic reticulum membrane organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endoplasmic reticulum membrane organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in endoplasmic reticulum-plasma membrane tethering IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phospholipid homeostasis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of phosphatidylinositol dephosphorylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in cell periphery HDA PubMed 
located_in cellular bud IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cortical endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum HDA PubMed 
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
tricalbin
NP_014312.1
  • Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER, the cell periphery and is enriched in bud membranes; mRNA is targeted to the bud tip

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    462411..465947
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182925.1 → NP_014312.1  TPA: tricalbin [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014312.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W192, P48231, Q45TY9
    UniProtKB/TrEMBL
    A6ZRZ3, N1NWL1
    Conserved Domains (1) summary
    COG5038
    Location:4 → 1178
    COG5038; Ca2+-dependent lipid-binding protein, contains C2 domain [General function prediction only]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P48231.1

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