LEM3 Lem3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: LEM3
Official Full Name: Lem3p
Primary source: SGD:S000005267
Locus tag: YNL323W
See related: AllianceGenome:SGD:S000005267; FungiDB:YNL323W; VEuPathDB:YNL323W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BRE3; ROS3
Summary: Contributes to aminophospholipid flippase activity and phosphatidylcholine flippase activity. Involved in cell surface receptor signaling pathway; phospholipid translocation; and regulation of vacuole organization. Located in endoplasmic reticulum and plasma membrane. Part of phospholipid-translocating ATPase complex. Orthologous to several human genes including TMEM30B (transmembrane protein 30B). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See LEM3 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (31944..33188)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.
Import of choline phospholipids into S. cerevisiae DeltaLem3 vector is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing that the proteins are orthologous.
Title: Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells.
Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains.
Title: Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae.
The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
Lem3p-Dnf1p complex may play a role distinct from its plasma membrane aminophospholipid translocase activity when it substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments.
Title: Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to aminophospholipid flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to phosphatidylcholine flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in aminophospholipid translocation	IEA Inferred from Electronic Annotation more info
involved_in cell surface receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vacuole organization	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of phospholipid-translocating ATPase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Lem3p

NP_014076.1

Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

31944..33188

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183161.1 → NP_014076.1 TPA: Lem3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014076.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0M3, P42838

UniProtKB/TrEMBL

A6ZSF8, B3LPF6, C7GWR9, C8ZGK2, G2WLE7, N1NWZ5

Conserved Domains (1) summary

COG5035
Location:11 → 414

CDC50; Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms]